ArticlePlant physiology2007
Expression of a constitutively activated plasma membrane H+-ATPase alters plant development and increases salt tolerance.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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81 citing papers in PubMed, 190 citations in OpenAlex.
- A conserved hexapeptide switch controls autoinhibition of plant cytosolic invertases.Nature plants · 2026Article
- Ustilago maydis Phosphatidic Acid and Phosphatidylserine-Targeting Secreted Lipase Lip3 Is Involved in Pathogenesis.Molecular plant pathology · 2026Article
- Genome-wide identification and salt stress response analysis of the WD40 protein family in Populus yunnanensis.BMC genomics · 2026Article
- PROTON ATPASE TRANSLOCATION CONTROL 1-mediated HPNAS nexus · 2025Article
- Melatonin confers saline-alkali tolerance in tomato by alleviating nitrosative damage and S-nitrosylation of H+-ATPase 2.The Plant cell · 2025Article
- Identification of candidate genes for drought tolerance in soybean through QTL mapping and gene expression analysis.Frontiers in genetics · 2025Article
- Identification and Characterization of miRNAs and lncRNAs Associated with Salinity Stress in Rice Panicles.International journal of molecular sciences · 2024Article
- Whole Genome Duplication Events Likely Contributed to the Aquatic Adaptive Evolution of Parkerioideae.Plants (Basel, Switzerland) · 2024Article
- Arabidopsis COP1 guides stomatal response in guard cells through pH regulation.Communications biology · 2024Article
- The Microbial Connection to Sustainable Agriculture.Plants (Basel, Switzerland) · 2023Review
- Membrane Proteins in Plant Salinity Stress Perception, Sensing, and Response.The Journal of membrane biology · 2023Review
- The KFrontiers in plant science · 2023Article
- Review
- Membrane Proteomic Profiling of Soybean Leaf and Root Tissues Uncovers Salt-Stress-Responsive Membrane Proteins.International journal of molecular sciences · 2022Article
- Functional Analysis of the Plasma Membrane HJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Histone acetyltransferase GCN5-mediated lysine acetylation modulates salt stress aadaption of Trichoderma.Applied microbiology and biotechnology · 2022Article
- Arabidopsis Plasma Membrane ATPase AHA5 Is Negatively Involved in PAMP-Triggered Immunity.International journal of molecular sciences · 2022Article
- Salt-Specific Gene Expression Reveals Elevated Auxin Levels inFrontiers in plant science · 2022Article
- The root apoplastic pH as an integrator of plant signaling.Frontiers in plant science · 2022Review
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plasma membrane proton pump ATPase (H(+)-ATPase) plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. Its activity is regulated by a C-terminal autoinhibitory domain that can be displaced by phosphorylation and the binding of regulatory 14-3-3 proteins, resulting in an activated enzyme. To better understand the physiological consequence of this activation, we have analyzed transgenic tobacco (Nicotiana tabacum) plants expressing either wild-type plasma membrane H(+)-ATPase4 (wtPMA4) or a PMA4 mutant lacking the autoinhibitory domain (DeltaPMA4), generating a constitutively activated enzyme. Plants showing 4-fold higher expression of wtPMA4 than untransformed plants did not display any unusual phenotype and their leaf and root external acidification rates were not modified, while their in vitro H(+)-ATPase activity was markedly increased. This indicates that, in vivo, H(+)-ATPase overexpression is compensated by down-regulation of H(+)-ATPase activity. In contrast, plants that expressed DeltaPMA4 were characterized by a lower apoplastic and external root pH, abnormal leaf inclination, and twisted stems, suggesting alterations in cell expansion. This was confirmed by in vitro leaf extension and curling assays. These data therefore strongly support a direct role of H(+)-ATPase in plant development. The DeltaPMA4 plants also displayed increased salt tolerance during germination and seedling growth, supporting the hypothesis that H(+)-ATPase is involved in salt tolerance.
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