ArticleGene therapy2007
The effect of the histological properties of tumors on transfection efficiency of electrically assisted gene delivery to solid tumors in mice.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts.Oncology reports · 2019Article
- Upregulation of DNA Sensors in B16.F10 Melanoma Spheroid Cells After Electrotransfer of pDNA.Technology in cancer research & treatment · 2018Article
- Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo.Radiology and oncology · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue.The Journal of membrane biology · 2015Article
- Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues.Journal of biomolecular screening · 2015Review
- The culture of cancer cell lines as tumorspheres does not systematically result in cancer stem cell enrichment.PloS one · 2014Article
- Muscle gene electrotransfer is increased by the antioxidant tempol in mice.Gene therapy · 2012Article
- Hyaluronidase and collagenase increase the transfection efficiency of gene electrotransfer in various murine tumors.Human gene therapy · 2012Article
- Gene transfer: how can the biological barriers be overcome?The Journal of membrane biology · 2010Article
- A statistical model for multidimensional irreversible electroporation cell death in tissue.Biomedical engineering online · 2010Article
- Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 2 - In vivo developments and present clinical applications.Biophysical reviews · 2009Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uniform DNA distribution in tumors is a prerequisite step for high transfection efficiency in solid tumors. To improve the transfection efficiency of electrically assisted gene delivery to solid tumors in vivo, we explored how tumor histological properties affected transfection efficiency. In four different tumor types (B16F1, EAT, SA-1 and LPB), proteoglycan and collagen content was morphometrically analyzed, and cell size and cell density were determined in paraffin-embedded tumor sections under a transmission microscope. To demonstrate the influence of the histological properties of solid tumors on electrically assisted gene delivery, the correlation between histological properties and transfection efficiency with regard to the time interval between DNA injection and electroporation was determined. Our data demonstrate that soft tumors with larger spherical cells, low proteoglycan and collagen content, and low cell density are more effectively transfected (B16F1 and EAT) than rigid tumors with high proteoglycan and collagen content, small spindle-shaped cells and high cell density (LPB and SA-1). Furthermore, an optimal time interval for increased transfection exists only in soft tumors, this being in the range of 5-15 min. Therefore, knowledge about the histology of tumors is important in planning electrogene therapy with respect to the time interval between DNA injection and electroporation.
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