ArticleBMC genomics2007
Definition of the zebrafish genome using flow cytometry and cytogenetic mapping.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 75 citations in OpenAlex.
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- The Promise of the Zebrafish Model for Parkinson's Disease: Today's Science and Tomorrow's Treatment.Frontiers in genetics · 2021Review
- Highly efficient synchronization of sheep skin fibroblasts at G2/M phase and isolation of sheep Y chromosomes by flow cytometric sorting.Scientific reports · 2020Article
- Atrazine exposure elicits copy number alterations in the zebrafish genome.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2017Article
- The genetic basis for ecological adaptation of the Atlantic herring revealed by genome sequencing.eLife · 2016Article
- Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones.Chromosoma · 2014Article
- Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor β1a (TGFβ1a).The Journal of biological chemistry · 2013Article
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- Integrating cytogenetics and genomics in comparative evolutionary studies of cichlid fish.BMC genomics · 2012Article
- Reassessment of genome size in turtle and crocodile based on chromosome measurement by flow karyotyping: close similarity to chicken.Biology letters · 2012Article
- Extensive genetic diversity and substructuring among zebrafish strains revealed through copy number variant analysis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Multiple sex-associated regions and a putative sex chromosome in zebrafish revealed by RAD mapping and population genomics.PloS one · 2012Article
- Article
- Construction and application of a zebrafish array comparative genomic hybridization platform.Genes, chromosomes & cancer · 2009Article
- Asymmetrical distribution of non-conserved regulatory sequences at PHOX2B is reflected at the ENCODE loci and illuminates a possible genome-wide trend.BMC genomics · 2009Article
- Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays.Nature protocols · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundThe zebrafish (Danio rerio) is an important vertebrate model organism system for biomedical research. The syntenic conservation between the zebrafish and human genome allows one to investigate the function of human genes using the zebrafish model. To facilitate analysis of the zebrafish genome, genetic maps have been constructed and sequence annotation of a reference zebrafish genome is ongoing. However, the duplicative nature of teleost genomes, including the zebrafish, complicates accurate assembly and annotation of a representative genome sequence. Cytogenetic approaches provide "anchors" that can be integrated with accumulating genomic data.
resultsHere, we cytogenetically define the zebrafish genome by first estimating the size of each linkage group (LG) chromosome using flow cytometry, followed by the cytogenetic mapping of 575 bacterial artificial chromosome (BAC) clones onto metaphase chromosomes. Of the 575 BAC clones, 544 clones localized to apparently unique chromosomal locations. 93.8% of these clones were assigned to a specific LG chromosome location using fluorescence in situ hybridization (FISH) and compared to the LG chromosome assignment reported in the zebrafish genome databases. Thirty-one BAC clones localized to multiple chromosomal locations in several different hybridization patterns. From these data, a refined second generation probe panel for each LG chromosome was also constructed.
conclusionThe chromosomal mapping of the 575 large-insert DNA clones allows for these clones to be integrated into existing zebrafish mapping data. An accurately annotated zebrafish reference genome serves as a valuable resource for investigating the molecular basis of human diseases using zebrafish mutant models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.