ArticleMolecular biology of the cell2007
Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis.The Journal of cell biology · 2023Article
- Autophagy, Acute Pancreatitis and the Metamorphoses of a Trypsinogen-Activating Organelle.Cells · 2022Review
- LAP-like non-canonical autophagy and evolution of endocytic vacuoles in pancreatic acinar cells.Autophagy · 2020Article
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- Stochastic modeling of nanoparticle internalization and expulsion through receptor-mediated transcytosis.Nanoscale · 2019Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- Intracellular rupture, exocytosis and actin interaction of endocytic vacuoles in pancreatic acinar cells: initiating events in acute pancreatitis.The Journal of physiology · 2018Article
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Concerted actions of distinct nonmuscle myosin II isoforms drive intracellular membrane remodeling in live animals.The Journal of cell biology · 2017Article
- Article
- Real-time insights into regulated exocytosis.Journal of cell science · 2017Review
- Liver kinase B1 regulates hepatocellular tight junction distribution and function in vivo.Hepatology (Baltimore, Md.) · 2016Article
- Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.Nature communications · 2015Article
- Imaging membrane remodeling during regulated exocytosis in live mice.Experimental cell research · 2015Review
- Insulin secretion from beta cells within intact islets: location matters.Clinical and experimental pharmacology & physiology · 2015Review
- Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles.Journal of cell science · 2015Article
- Synaptotagmin-7 links fusion-activated Ca²⁺ entry and fusion pore dilation.Journal of cell science · 2014Article
- Two distinct modes of exocytotic fusion pore expansion in large astrocytic vesicles.The Journal of biological chemistry · 2013Article
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Regulated exocytosis: novel insights from intravital microscopy.Traffic (Copenhagen, Denmark) · 2012Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Loss of granule content during exocytosis requires the opening of a fusion pore between the secretory granule and plasma membrane. In a variety of secretory cells, this fusion pore has now been shown to subsequently close. However, it is still unclear how pore closure is physiologically regulated and contentious as to how closure relates to granule content loss. Here, we examine the behavior of the fusion pore during zymogen granule exocytosis in pancreatic acinar cells. By using entry of high-molecular-weight dyes from the extracellular solution into the granule lumen, we show that the fusion pore has a diameter of 29-55 nm. We further show that by 5 min after granule fusion, many granules have a closed fusion pore with evidence indicating that pore closure is a prelude to endocytosis and that in granules with a closed fusion pore the chymotrypsinogen content is low. Finally, we show that latrunculin B treatment promotes pore closure, suggesting F-actin affects pore dynamics. Together, our data do not support the classical view in acinar cells that exocytosis ends with granule collapse. Instead, for many granules the fusion pore closes, probably as a transition to endocytosis, and likely involving an F-actin-dependent mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.