ArticleMolecular biology of the cell2007
Rho/Rho-associated kinase-II signaling mediates disassembly of epithelial apical junctions.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 153 citations in OpenAlex.
- Hyaluronan and layilin mediate loss of airway epithelial barrier function induced by cigarette smoke by decreasing E-cadherin.The Journal of biological chemistry · 2012Trial
- Deficiency of ZFP36L1 and ZFP36L2 impairs liver homeostasis and initiates cholestatic liver injury.Hepatology communications · 2026Article
- Molecular Motors in Blood-Brain Barrier Maintenance by Astrocytes.Brain sciences · 2025Review
- Plakophilin 4 controls the spatio-temporal activity of RhoA at adherens junctions to promote cortical actin ring formation and tissue tension.Cellular and molecular life sciences : CMLS · 2024Article
- Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndrome.Journal of translational medicine · 2023Article
- Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2.iScience · 2023Article
- Cytokinesis machinery promotes cell dissociation from collectively migrating strands in confinement.Science advances · 2023Article
- Therapeutic Validation of GEF-H1 Using a De Novo Designed Inhibitor in Models of Retinal Disease.Cells · 2022Article
- Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells.Cellular microbiology · 2021Article
- A narrative review of changes in microvascular permeability after burn.Annals of translational medicine · 2021Review
- Apical polarity proteins recruit the RhoGEF Cysts to promote junctional myosin assembly.The Journal of cell biology · 2019Article
- The cerebral cavernous malformation disease causing gene KRIT1 participates in intestinal epithelial barrier maintenance and regulation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Cell polarization: From epithelial cells to odontoblasts.European journal of cell biology · 2019Review
- Phosphorylation hotspot in the C-terminal domain of occludin regulates the dynamics of epithelial junctional complexes.Journal of cell science · 2018Article
- Lysophosphatidic Acid Receptor 1 Is Important for Intestinal Epithelial Barrier Function and Susceptibility to Colitis.The American journal of pathology · 2018Article
- The role of microtubules in the regulation of epithelial junctions.Tissue barriers · 2018Review
- mTORC1 loss impairs epidermal adhesion via TGF-β/Rho kinase activation.The Journal of clinical investigation · 2017Article
- Disruption of the epithelial barrier during intestinal inflammation: Quest for new molecules and mechanisms.Biochimica et biophysica acta. Molecular cell research · 2017Review
- Intestinal epithelial claudins: expression and regulation in homeostasis and inflammation.Annals of the New York Academy of Sciences · 2017Review
- Substrate roughness induces the development of defective E-cadherin junctions in human gingival keratinocytes.Journal of periodontal & implant science · 2017Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Apical junctional complex (AJC) plays a vital role in regulation of epithelial barrier function. Disassembly of the AJC is observed in diverse physiological and pathological states; however, mechanisms governing this process are not well understood. We previously reported that the AJC disassembly is driven by the formation of apical contractile acto-myosin rings. In the present study, we analyzed the signaling pathways regulating acto-myosin-dependent disruption of AJC by using a model of extracellular calcium depletion. Pharmacological inhibition analysis revealed a critical role of Rho-associated kinase (ROCK) in AJC disassembly in calcium-depleted epithelial cells. Furthermore, small interfering RNA (siRNA)-mediated knockdown of ROCK-II, but not ROCK-I, attenuated the disruption of the AJC. Interestingly, AJC disassembly was not dependent on myosin light chain kinase and myosin phosphatase. Calcium depletion resulted in activation of Rho GTPase and transient colocalization of Rho with internalized AJC proteins. Pharmacological inhibition of Rho prevented AJC disassembly. Additionally, Rho guanine nucleotide exchange factor (GEF)-H1 translocated to contractile F-actin rings after calcium depletion, and siRNA-mediated depletion of GEF-H1 inhibited AJC disassembly. Thus, our findings demonstrate a central role of the GEF-H1/Rho/ROCK-II signaling pathway in the disassembly of AJC in epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.