ArticleJournal of clinical microbiology2007
Comparison of four methods using throat swabs to confirm rubella virus infection.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Measles and rubella: From global health challenges to advancements in molecular diagnostics in the elimination era.Molecular therapy. Nucleic acids · 2025Review
- Exogenous Rubella Virus Capsid Proteins Enhance Virus Genome Replication.Pathogens (Basel, Switzerland) · 2022Article
- Importation and circulation of rubella virus lineages 1E-L2 and 2B-L2c between 2018 and 2021 in China: Virus evolution and spatial-temporal transmission characteristics.Virus evolution · 2022Article
- Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays.Clinical microbiology reviews · 2019Review
- Varicella zoster and fever rash surveillance in Lao People's Democratic Republic.BMC infectious diseases · 2019Article
- Association of Ocular Inflammation and Rubella Virus Persistence.JAMA ophthalmology · 2019Article
- Seroprevalence of rubella virus antibodies among pregnant women in the Center and South-West regions of Cameroon.PloS one · 2019Article
- Rubella seroprevalence among pregnant women in Beijing, China.BMC infectious diseases · 2018Article
- Progress towards rubella elimination after implementation of rubella immunization for over 20 years in Shandong province, China.Scientific reports · 2017Article
- Analysis of complete genomes of the rubella virus genotypes 1E and 2B which circulated in China, 2000-2013.Scientific reports · 2016Article
- Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogens.Genome medicine · 2016Article
- Identification of microRNAs in Throat Swab as the Biomarkers for Diagnosis of Influenza.International journal of medical sciences · 2016Article
- Rubella.Lancet (London, England) · 2015Review
- Evolutionary analysis of rubella viruses in mainland China during 2010-2012: endemic circulation of genotype 1E and introductions of genotype 2B.Scientific reports · 2015Article
- Article
- Phylogenetic analysis of rubella viruses identified in Uganda, 2003-2012.Journal of medical virology · 2014Article
- Rubella epidemic caused by genotype 1E rubella viruses in Beijing, China, in 2007-2011.Virology journal · 2013Article
- Emergence and continuous evolution of genotype 1E rubella viruses in China.Journal of clinical microbiology · 2012Article
- Rubella epidemics and genotypic distribution of the rubella virus in Shandong Province, China, in 1999-2010.PloS one · 2012Article
- Rubella virus genotypes in the People's Republic of China between 1979 and 2007: a shift in endemic viruses during the 2001 Rubella Epidemic.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laboratory tests are essential for confirming sporadic cases and outbreaks of rubella. Detection of rubella virus is often necessary to confirm rubella cases and to identify specimens to be used to characterize wild-type rubella viruses. The sensitivities of four methods for detecting rubella virus infection using throat swabs, which had been collected in Henan and Anhui provinces in China, were evaluated. The methods used were reverse transcription (RT)-PCR followed by Southern hybridization using RNA extracted directly from clinical specimens, virus growth in tissue culture followed by virus detection by RT-PCR, low-background immunofluorescence in infected tissue culture cells using monoclonal antibodies to the structural proteins of rubella virus, and a replicon-based method of detecting infectious virus. Among these four methods, direct RT-PCR followed by hybridization was the most sensitive method; the replicon-based method was the least difficult to perform.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.