ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2007
Fluorescence in situ hybridization using an old world monkey Y chromosome specific probe combined with immunofluorescence staining on rhesus monkey tissues.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 5 citations in OpenAlex.
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- Ovulation and extra-ovarian origin of ovarian cancer.Scientific reports · 2014Article
- Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Review
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6 authors at 2 institutions in 2 countries.
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Abstract
To date, there is no commercially available Y chromosome probe that can be used for fluorescence in situ hybridization (FISH) for the male rhesus monkey. We have recently generated a probe for FISH with high specificity to the short arm of the rhesus monkey Y chromosome. In this study, we further describe a method that keeps the integrity of tissue-specific antigenic structures for immunofluorescence staining subsequent to FISH on paraffin-embedded rhesus monkey tissues. We have examined this technique in combination with an epithelial cell-specific marker, cytokeratin 8/18 (CK8/18), on various tissues, including jejunum, liver, kidney, and pancreas. CK8/18 and Y chromosome signals were distinctly seen simultaneously on epithelial cells from the same tissue section from male but not female monkeys. These studies indicate that our FISH immunofluorescence technique can be reliably used to identify and phenotype male cells in paraffin-embedded rhesus monkey tissues.
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