ArticlePloS one2007
Expression, processing, and localization of PmpD of Chlamydia trachomatis Serovar L2 during the chlamydial developmental cycle.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- Addressing Challenges inMedicina (Kaunas, Lithuania) · 2024Review
- Sequence, structure prediction, and epitope analysis of the polymorphic membrane protein family in Chlamydia trachomatis.PloS one · 2024Article
- The secreted host-cell protein clusterin interacts with PmpD and promotesFrontiers in cellular and infection microbiology · 2024Article
- Heterologous Display ofMembranes · 2023Article
- Whole Genome Sequencing of aCurrent issues in molecular biology · 2023Article
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- A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains.Pathogens and disease · 2019Article
- Chlamydial Infection From Outside to Inside.Frontiers in microbiology · 2019Review
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- In silico prediction of T- and B-cell epitopes in PmpD: First step towards to the design of a Chlamydia trachomatis vaccine.Biomedical journal · 2018Article
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- Article
- Chlamydial polymorphic membrane proteins: regulation, function and potential vaccine candidates.Virulence · 2016Review
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- Engineered phage-based therapeutic materials inhibit Chlamydia trachomatis intracellular infection.Biomaterials · 2012Article
- Processing of Chlamydia abortus polymorphic membrane protein 18D during the chlamydial developmental cycle.PloS one · 2012Article
- Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms.Molecular microbiology · 2011Article
- Altered developmental expression of polymorphic membrane proteins in penicillin-stressed Chlamydia trachomatis.Cellular microbiology · 2011Article
- Genome sequencing of recent clinical Chlamydia trachomatis strains identifies loci associated with tissue tropism and regions of apparent recombination.Infection and immunity · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWhile families of polymorphic membrane protein (pmp) genes have been identified in several Chlamydia species, their function remains mostly unknown. These proteins are of great interest, however, because of their location in the outer membrane and possible role in chlamydial virulence. METHODOLOGY/PRINCIPAL FINDING: We analyzed the relative transcription of the pmpD gene, a member of the pmp gene family in C. trachomatis serovar L2, and its protein product translation and processing during the chlamydial developmental cycle. By real-time reverse transcription polymerase chain reaction, the pmpD gene was found to be upregulated at 16 to 24 four hours after infection. Using polyclonal antibodies generated against the predicted passenger domain of PmpD, we demonstrated that it is initially localized on the surface of reticulate bodies, followed by its secretion outside Chlamydia starting at 24 hours after infection. In elementary bodies, we found a approximately 157 kDa PmpD only inside the cell. Both events, the upregulation of pmpD gene transcription and PmpD protein processing and secretion, are coincidental with the period of replication and differentiation of RBs into EBs. We also demonstrated that, in the presence of penicillin, the cleavage and secretion of the putative passenger domain was suppressed. CONCLUSION/SIGNIFICANCE: Our results are in agreement with the general concept that PmpD is an autotransporter protein which is post-translationally processed and secreted in the form of the putative passenger domain outside Chlamydia at mid- to- late point after infection, coinciding with the development of RBs into EBs.
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