ArticlePlant cell reports2007
Regeneration of transgenic plants from two indica rice (Oryza sativa L.) cultivars using shoot apex explants.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 36 citations in OpenAlex.
- Rapid Agrobacterium-mediated transformation and high-efficiency regeneration of finger millet (Eleusine coracana) for crop improvement.Transgenic research · 2026Article
- Direct Shoot Regeneration from the Finger Millet's In Vitro-Derived Shoot Apex and Genetic Fidelity Study with ISSR Markers.Biotech (Basel (Switzerland)) · 2025Article
- Review
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- One-Step Regeneration of Hairy Roots to Induce High Tanshinone Plants inFrontiers in plant science · 2022Article
- Role of activated charcoal and amino acids in developing an efficient regeneration system for foxtail millet (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Finger Millet [Frontiers in plant science · 2018Review
- An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars.Plant methods · 2011Article
- Agrobacterium-mediated transformation of finger millet (Eleusine coracana (L.) Gaertn.) using shoot apex explants.Plant cell reports · 2011Article
- Tissue specific response of Agrobacterium tumefaciens attachment to Sorghum bicolor (L) Moench.Physiology and molecular biology of plants : an international journal of functional plant biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have established a reproducible procedure for transformation of shoot apices and regeneration of transgenic plants for two indica rice cultivars, white ponni (WP) and Pusa Basmathi 1 (PB 1). Four-day-old shoot apex explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA 101 harbouring a binary plasmid pRIT1. The vector contained an improved hygromycin phosphotransferase (hpt) gene for hygromycin resistance driven by actin 1 promoter and the reporter gene beta-glucuronidase intron (INT-GUS) controlled by CaMV 35S promoter. Rice shoots were induced on media containing 0.1 mg/l napthalene acetic acid (NAA), 1.0 mg/l kinetin (kn), 1.0 mg/l N(6)-benzyleaminopurin (BAP), 300 mg/l casaminoacid, 500 mg/l proline, 50 mg/l hygromycin and 500 mg/l cefotaxime. Transgenic plants were raised in pots and seeds were collected. Histochemical and polymerase chain reaction (PCR) analyses of field established transgenic rice plants and their offsprings confirmed the presence of GUS gene. Integration of T-DNA into the genome of putative transgenics was further confirmed by southern analysis. The transformation efficiency of WP was found to be ranging from 5.6 to 6.2% whereas in the case of PB1, it was from 7 to 8%. Progeny analysis of these plants showed a pattern of classical Mendelian inheritance for both hpt and GUS gene.
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