ArticleBMC microbiology2007
Interaction of salivary alpha-amylase and amylase-binding-protein A (AbpA) of Streptococcus gordonii with glucosyltransferase of S. gordonii and Streptococcus mutans.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Modulating the Microbiome for Crohn's Disease Treatment.Gastroenterology · 2023Review
- Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface.Frontiers in cellular and infection microbiology · 2022Article
- Surface Effect of Nano-Roughened Yttria-Doped Zirconia on Salivary Protein Adhesion.Materials (Basel, Switzerland) · 2021Article
- Bioinspired caries preventive strategy via customizable pellicles of saliva-derived protein/peptide constructs.Scientific reports · 2021Article
- Amylases: Biofilm Inducer or Biofilm Inhibitor?Frontiers in cellular and infection microbiology · 2021Review
- Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.PloS one · 2021Article
- Murine Salivary Amylase Protects AgainstFrontiers in physiology · 2021Article
- Sulfated vizantin causes detachment of biofilms composed mainly of the genus Streptococcus without affecting bacterial growth and viability.BMC microbiology · 2020Article
- Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins.Biological research · 2020Article
- Article
- Effects of soybean milk, chitosan, and anti-Streptococcus mutans IgY in malnourished rats' dental biofilm and the IgY persistency in saliva.Interventional medicine & applied science · 2015Article
- Dynamics of the Streptococcus gordonii Transcriptome in Response to Medium, Salivary α-Amylase, and Starch.Applied and environmental microbiology · 2015Article
- Structure of amylase-binding protein A of Streptococcus gordonii: a potential receptor for human salivary α-amylase enzyme.Protein science : a publication of the Protein Society · 2015Article
- Mass Spectrometric Analysis of Whole Secretome and Amylase-precipitated Secretome Proteins fromJournal of proteomics & bioinformatics · 2014Article
- Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary α-amylase binding to oral streptococci.Applied and environmental microbiology · 2013Review
- Effect of starch and amylase on the expression of amylase-binding protein A in Streptococcus gordonii.Molecular oral microbiology · 2012Article
- Streptococcus mutans out-competes Streptococcus gordonii in vivo.Journal of dental research · 2012Article
- Response of fatty acid synthesis genes to the binding of human salivary amylase by Streptococcus gordonii.Applied and environmental microbiology · 2012Article
- Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms.Caries research · 2011Review
- Salivary α-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells.Journal of experimental & clinical cancer research : CR · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundGlucosyltransferases (Gtfs), enzymes that produce extracellular glucans from dietary sucrose, contribute to dental plaque formation by Streptococcus gordonii and Streptococcus mutans. The alpha-amylase-binding protein A (AbpA) of S. gordonii, an early colonizing bacterium in dental plaque, interacts with salivary amylase and may influence dental plaque formation by this organism. We examined the interaction of amylase and recombinant AbpA (rAbpA), together with Gtfs of S. gordonii and S. mutans.
resultsThe addition of salivary alpha-amylase to culture supernatants of S. gordonii precipitated a protein complex containing amylase, AbpA, amylase-binding protein B (AbpB), and the glucosyltransferase produced by S. gordonii (Gtf-G). rAbpA was expressed from an inducible plasmid, purified from Escherichia coli and characterized. Purified rAbpA, along with purified amylase, interacted with and precipitated Gtfs from culture supernatants of both S. gordonii and S. mutans. The presence of amylase and/or rAbpA increased both the sucrase and transferase component activities of S. mutans Gtf-B. Enzyme-linked immunosorbent assay (ELISA) using anti-Gtf-B antibody verified the interaction of rAbpA and amylase with Gtf-B. A S. gordonii abpA-deficient mutant showed greater biofilm growth under static conditions than wild-type in the presence of sucrose. Interestingly, biofilm formation by every strain was inhibited in the presence of saliva.
conclusionThe results suggest that an extracellular protein network of AbpA-amylase-Gtf may influence the ecology of oral biofilms, likely during initial phases of colonization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.