ArticleProceedings of the National Academy of Sciences of the United States of America2007
Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 102 citations in OpenAlex.
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- Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes.NPJ science of food · 2026Article
- Trunk muscle differentiation-a lesson from the Chinese fire-bellied newt (Hypselotriton orientalis).Protoplasma · 2025Article
- SIX1+PAX3+ identify a progenitor for myogenic lineage commitment from hPSCs.Development (Cambridge, England) · 2023Article
- Overlapping functions of SIX homeoproteins during embryonic myogenesis.PLoS genetics · 2023Article
- The circadian E3 ligase FBXL21 regulates myoblast differentiation and sarcomere architecture via MYOZ1 ubiquitination and NFAT signaling.PLoS genetics · 2022Article
- Effects of high-molecular-weight polyvinyl chloride on Xenopus laevis adults and embryos: the mRNA expression profiles of Myf5, Esr1, Bmp4, Pax6, and Hsp70 genes during early embryonic development.Environmental science and pollution research international · 2022Article
- Muscle Enriched Lamin Interacting Protein (Cells · 2021Article
- Time-dependent Pax3-mediated chromatin remodeling and cooperation with Six4 and Tead2 specify the skeletal myogenic lineage in developing mesoderm.PLoS biology · 2019Article
- Location, Location, Location: Signals in Muscle Specification.Journal of developmental biology · 2018Review
- Review
- Myogenic progenitor specification from pluripotent stem cells.Seminars in cell & developmental biology · 2017Review
- Sine Oculis Homeobox Homolog 1 Regulates Mitochondrial Apoptosis Pathway Via Caspase-7 In Gastric Cancer Cells.Journal of Cancer · 2017Article
- MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis.Nucleic acids research · 2016Article
- Six1 induces protein synthesis signaling expression in duck myoblasts mainly via up-regulation of mTOR.Genetics and molecular biology · 2016Article
- Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle.Skeletal muscle · 2016Article
- Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer.Biology open · 2015Article
- Regulation of male sex determination: genital ridge formation and Sry activation in mice.Cellular and molecular life sciences : CMLS · 2014Review
- MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1.Journal of cell science · 2014Article
- Muscle satellite cell heterogeneity and self-renewal.Frontiers in cell and developmental biology · 2014Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myf5, a member of the myogenic regulatory factor family, plays a major role in determining myogenic cell fate at the onset of skeletal muscle formation in the embryo. Spatiotemporal control of its expression during development requires multiple enhancer elements spread over >100 kb at the Myf5 locus. Transcription in embryonic limbs is regulated by a 145-bp element located at -57.5 kb from the Myf5 gene. In the present study we show that Myf5 expression is severely impaired in the limb buds of Six1(-/-) and Six1(-/-)Six4(-/+) mouse mutants despite the presence of myogenic progenitor cells. The 145-bp regulatory element contains a sequence that binds Six1 and Six4 in electromobility shift assays in vitro and in chromatin immunoprecipitation assays with embryonic extracts. We further show that Six1 is able to transactivate a reporter gene under the control of this sequence. In vivo functionality of the Six binding site is demonstrated by transgenic analysis. Mutation of this site impairs reporter gene expression in the limbs and in mature somites where the 145-bp regulatory element is also active. Six1/4 therefore regulate Myf5 transcription, together with Pax3, which was previously shown to be required for the activity of the 145-bp element. Six homeoproteins, which also directly regulate the myogenic differentiation gene Myogenin and lie genetically upstream of Pax3, thus control hypaxial myogenesis at multiple levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.