ArticleProceedings of the National Academy of Sciences of the United States of America2007
Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 94 citations in OpenAlex.
- Building peroxisomes: perspectives on plant peroxins.Biochemical Society transactions · 2026Review
- PEX11 mediates intralumenal vesicle formation in peroxisomes.Nature communications · 2026Article
- Proteostasis and protein quality control in chloroplasts: mechanisms and novel insights related to protein mislocalization.Journal of experimental botany · 2025Review
- Identifying and characterizing a missing peroxin-PEX8-in Arabidopsis thaliana.The Plant cell · 2025Article
- Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis.Applied microbiology and biotechnology · 2022Article
- Plant peroxisome proteostasis-establishing, renovating, and dismantling the peroxisomal proteome.Essays in biochemistry · 2022Review
- Translating theFrontiers in cell and developmental biology · 2022Review
- Anaerobic peroxisomes inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile AlgaFrontiers in cell and developmental biology · 2020Article
- PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.Journal of integrative plant biology · 2019Article
- APlant direct · 2019Article
- AProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- Disparate peroxisome-related defects in Arabidopsis pex6 and pex26 mutants link peroxisomal retrotranslocation and oil body utilization.The Plant journal : for cell and molecular biology · 2017Article
- The PEX1 ATPase Stabilizes PEX6 and Plays Essential Roles in Peroxisome Biology.Plant physiology · 2017Article
- Plant peroxisomes: recent discoveries in functional complexity, organelle homeostasis, and morphological dynamics.Current opinion in plant biology · 2016Review
- The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures.Rice (New York, N.Y.) · 2016Article
- E3 ubiquitin ligase SP1 regulates peroxisome biogenesis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Genetic Interactions between PEROXIN12 and Other Peroxisome-Associated Ubiquitination Components.Plant physiology · 2016Article
- The Roles of β-Oxidation and Cofactor Homeostasis in Peroxisome Distribution and Function in Arabidopsis thaliana.Genetics · 2016Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glyoxysomes are a subclass of peroxisomes involved in lipid mobilization. Two distinct peroxisomal targeting signals (PTSs), the C-terminal PTS1 and the N-terminal PTS2, are defined. Processing of the PTS2 on protein import is conserved in higher eukaryotes. The cleavage site typically contains a Cys at P1 or P2. We purified the glyoxysomal processing protease (GPP) from the fat-storing cotyledons of watermelon (Citrullus vulgaris) by column chromatography, preparative native isoelectric focusing, and 2D PAGE. The GPP appears in two forms, a 72-kDa monomer and a 144-kDa dimer, which are in equilibrium with one another. The equilibrium is shifted on Ca(2+) removal toward the monomer and on Ca(2+) addition toward the dimer. The monomer is a general degrading protease and is activated by denatured proteins. The dimer constitutes the processing protease because the substrate specificity proven for the monomer (Phi-Arg/Lys downward arrow) is different from the processing substrate specificity (Cys-Xxx downward arrow/Xxx-Cys downward arrow) found with the mixture of monomer and dimer. The Arabidopsis genome analysis disclosed three proteases predicted to be in peroxisomes, a Deg-protease, a pitrilysin-like metallopeptidase, and a Lon-protease. Specific antibodies against the peroxisomal Deg-protease from Arabidopsis (Deg15) identify the watermelon GPP as a Deg15. A knockout mutation in the DEG15 gene of Arabidopsis (At1g28320) prevents processing of the glyoxysomal malate dehydrogenase precursor to the mature form. Thus, the GPP/Deg15 belongs to a group of trypsin-like serine proteases with Escherichia coli DegP as a prototype. Nevertheless, the GPP/Deg15 possesses specific characteristics and is therefore a new subgroup within the Deg proteases.
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