ArticleThe Journal of general physiology2007
Saturation and microsecond gating of current indicate depletion-induced instability of the MaxiK selectivity filter.
Article in The Journal of general physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Similarity of the ion occupations of KChannels (Austin, Tex.) · 2026Article
- Diffusion Limitations and Translocation Barriers in Atomically Thin Biomimetic Pores.Entropy (Basel, Switzerland) · 2020Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Cell Cycle-Dependent Expression of Bk Channels in Human Mesenchymal Endometrial Stem Cells.Scientific reports · 2019Article
- Site-specific ion occupation in the selectivity filter causes voltage-dependent gating in a viral KScientific reports · 2018Article
- Article
- Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter.FEBS letters · 2017Article
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Article
- Stabilization of the conductive conformation of a voltage-gated K+ (Kv) channel: the lid mechanism.The Journal of biological chemistry · 2013Article
- Lack of negative slope in I-V plots for BK channels at positive potentials in the absence of intracellular blockers.The Journal of general physiology · 2013Article
- Ca2+ block and flickering both contribute to the negative slope of the IV curve in BK channels.The Journal of general physiology · 2013Article
- Modulation of enrofloxacin binding in OmpF by Mg2+ as revealed by the analysis of fast flickering single-porin current.The Journal of general physiology · 2012Article
- A permeation theory for single-file ion channels: one- and two-step models.The Journal of chemical physics · 2011Article
- Coupling and cooperativity in voltage activation of a limited-state BK channel gating in saturating Ca2+.The Journal of general physiology · 2010Article
- Separation of heteromeric potassium channel Kcv towards probing subunit composition-regulated ion permeation and gating.FEBS letters · 2010Article
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- Fast and slow gating are inherent properties of the pore module of the K+ channel Kcv.The Journal of general physiology · 2009Article
- Interference of shot noise of open-channel current with analysis of fast gating: patchers do not (Yet) have to care.The Journal of membrane biology · 2009Article
- Four-mode gating model of fast inactivation of sodium channel Nav1.2a.Pflugers Archiv : European journal of physiology · 2008Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Patch clamp experiments on single MaxiK channels expressed in HEK293 cells were performed with a high temporal resolution (50-kHz filter) in symmetrical solutions with 50, 150, or 400 mM KCl and 2.5 mM CaCl(2) and 2.5 mM MgCl(2). At membrane potentials >+100 mV, the single-channel current showed a negative slope resistance, concomitantly with a flickery block, which was not influenced by Ca(2+) or Mg(2+). The analysis of the amplitude histograms by beta distributions revealed that current in this voltage range was reduced by two effects: rate limitation at the cytosolic side of the pore and gating with rate constants 10-20-fold higher than the cutoff frequency of the filter (i.e., dwell times in the microsecond range). The data were analyzed in terms of a model that postulates a coupling between both effects; if the voltage over the selectivity filter withdraws ions from the cavity at a higher rate than that of refilling from the cytosol, the selectivity filter becomes instable because of ion depletion, and current is interrupted by the resulting flickering. The fit of the IV curves revealed a characteristic voltage of 35 mV. In contrast, the voltage dependence of the gating factor R, i.e., the ratio between true and apparent single-channel current, could be fitted by exponentials with a characteristic voltage of 60 mV, suggesting that only part of the transmembrane potential is felt by the flux through the selectivity filter.
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