ArticleThe Journal of general physiology2007
Physiological properties of rod photoreceptor cells in green-sensitive cone pigment knock-in mice.
Article in The Journal of general physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 68 citations in OpenAlex.
- Minimal Mimics and Maps of Natural Light for Mammals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Spatiotemporal dynamics of ecto-5'-nucleotidase (CD73) in mouse retina under physiological conditions.Development (Cambridge, England) · 2026Article
- Retinal Origins of Circadian Photoregulation's Specialized Dynamic Range and Temporal Integration.bioRxiv : the preprint server for biology · 2025Article
- Human opsin restoration by histone methylation using methyltransferase fusion protein SETD7-dCas9.Molecular therapy. Nucleic acids · 2025Article
- Optimization of HITI-Mediated Gene Insertion for Rhodopsin and Peripherin-2 in Mouse Rod Photoreceptors: Targeting Dominant Retinitis Pigmentosa.Investigative ophthalmology & visual science · 2024Article
- Light induces a rapid increase in cAMP and activates PKA in rod outer segments of the frog retina.The Journal of general physiology · 2024Article
- Preclinical evaluation of ADVM-062, a novel intravitreal gene therapy vector for the treatment of blue cone monochromacy.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Adaptation of Antarctic Icefish Vision to Extreme Environments.Molecular biology and evolution · 2023Article
- Glucose uptake by GLUT1 in photoreceptors is essential for outer segment renewal and rod photoreceptor survival.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Gene Therapy inHuman gene therapy · 2022Article
- dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.Nature protocols · 2022Review
- Phototransduction in Anuran Green Rods: Origins of Extra-Sensitivity.International journal of molecular sciences · 2021Article
- Evolutionary adaptation of visual pigments in geckos for their photic environment.Science advances · 2021Article
- A kinetic analysis of mouse rod and cone photoreceptor responses.The Journal of physiology · 2020Article
- A gene therapy for inherited blindness using dCas9-VPR-mediated transcriptional activation.Science advances · 2020Article
- Investigating the CaScientific reports · 2018Article
- Adaptation of cone pigments found in green rods for scotopic vision through a single amino acid mutation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The evolution of rod photoreceptors.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Spontaneous activation of visual pigments in relation to openness/closedness of chromophore-binding pocket.eLife · 2017Article
- Why are rods more sensitive than cones?The Journal of physiology · 2016Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rod and cone photoreceptor cells that are responsible for scotopic and photopic vision, respectively, exhibit photoresponses different from each other and contain similar phototransduction proteins with distinctive molecular properties. To investigate the contribution of the different molecular properties of visual pigments to the responses of the photoreceptor cells, we have generated knock-in mice in which rod visual pigment (rhodopsin) was replaced with mouse green-sensitive cone visual pigment (mouse green). The mouse green was successfully transported to the rod outer segments, though the expression of mouse green in homozygous retina was approximately 11% of rhodopsin in wild-type retina. Single-cell recordings of wild-type and homozygous rods suggested that the flash sensitivity and the single-photon responses from mouse green were three to fourfold lower than those from rhodopsin after correction for the differences in cell volume and levels of several signal transduction proteins. Subsequent measurements using heterozygous rods expressing both mouse green and rhodopsin E122Q mutant, where these pigments in the same rod cells can be selectively irradiated due to their distinctive absorption maxima, clearly showed that the photoresponse of mouse green was threefold lower than that of rhodopsin. Noise analysis indicated that the rate of thermal activations of mouse green was 1.7 x 10(-7) s(-1), about 860-fold higher than that of rhodopsin. The increase in thermal activation of mouse green relative to that of rhodopsin results in only 4% reduction of rod photosensitivity for bright lights, but would instead be expected to severely affect the visual threshold under dim-light conditions. Therefore, the abilities of rhodopsin to generate a large single photon response and to retain high thermal stability in darkness are factors that have been necessary for the evolution of scotopic vision.
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