ArticlePLoS pathogens2007
Gene expression profiles of Chlamydophila pneumoniae during the developmental cycle and iron depletion-mediated persistence.
Article in PLoS pathogens, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 89 citations in OpenAlex.
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- A Functional Yeast-Based Screen Identifies the Host Microtubule Cytoskeleton as a Target of NumerousInternational journal of molecular sciences · 2023Article
- Persistence of obligate intracellular pathogens: alternative strategies to overcome host-specific stresses.Frontiers in cellular and infection microbiology · 2023Review
- Transcriptional Landscape ofMicroorganisms · 2020Article
- Molecular causes of an evolutionary shift along the parasitism-mutualism continuum in a bacterial symbiont.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Insights Into aFrontiers in cellular and infection microbiology · 2020Article
- Transcriptomic Changes ofFrontiers in cellular and infection microbiology · 2019Article
- Ptr/CTL0175 Is Required for the Efficient Recovery ofFrontiers in microbiology · 2019Article
- Virulence-related comparative transcriptomics of infectious and non-infectious chlamydial particles.BMC genomics · 2018Article
- Review
- Ironing Out the Unconventional Mechanisms of Iron Acquisition and Gene Regulation inFrontiers in cellular and infection microbiology · 2017Review
- A working model for the type III secretion mechanism in Chlamydia.Microbes and infection · 2016Review
- The Impact of Protein Phosphorylation on Chlamydial Physiology.Frontiers in cellular and infection microbiology · 2016Review
- Article
- Following the Footsteps of Chlamydial Gene Regulation.Molecular biology and evolution · 2015Article
- The Rsb Phosphoregulatory Network Controls Availability of the Primary Sigma Factor in Chlamydia trachomatis and Influences the Kinetics of Growth and Development.PLoS pathogens · 2015Article
- Phosphoproteomic analysis of the Chlamydia caviae elementary body and reticulate body forms.Microbiology (Reading, England) · 2015Article
- Modulation of host signaling and cellular responses by Chlamydia.Cell communication and signaling : CCS · 2013Review
- Laboratory diagnosis of persistent human chlamydial infection.Frontiers in cellular and infection microbiology · 2013Review
- Intracellular survival and persistence of Chlamydia muridarum is determined by macrophage polarization.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The obligate intracellular, gram-negative bacterium Chlamydophila pneumoniae (Cpn) has impact as a human pathogen. Little is known about changes in the Cpn transcriptome during its biphasic developmental cycle (the acute infection) and persistence. The latter stage has been linked to chronic diseases. To analyze Cpn CWL029 gene expression, we designed a pathogen-specific oligo microarray and optimized the extraction method for pathogen RNA. Throughout the acute infection, ratio expression profiles for each gene were generated using 48 h post infection as a reference. Based on these profiles, significantly expressed genes were separated into 12 expression clusters using self-organizing map clustering and manual sorting into the "early", "mid", "late", and "tardy" cluster classes. The latter two were differentiated because the "tardy" class showed steadily increasing expression at the end of the cycle. The transcriptome of the Cpn elementary body (EB) and published EB proteomics data were compared to the cluster profile of the acute infection. We found an intriguing association between "late" genes and genes coding for EB proteins, whereas "tardy" genes were mainly associated with genes coding for EB mRNA. It has been published that iron depletion leads to Cpn persistence. We compared the gene expression profiles during iron depletion-mediated persistence with the expression clusters of the acute infection. This led to the finding that establishment of iron depletion-mediated persistence is more likely a mid-cycle arrest in development rather than a completely distinct gene expression pattern. Here, we describe the Cpn transcriptome during the acute infection, differentiating "late" genes, which correlate to EB proteins, and "tardy" genes, which lead to EB mRNA. Expression profiles during iron mediated-persistence led us to propose the hypothesis that the transcriptomic "clock" is arrested during acute mid-cycle.
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