ArticleWorld journal of gastroenterology2007
Isolation of Kupffer cells and their suppressive effects on T lymphocyte growth in rat orthotopic liver transplantation.
Article in World journal of gastroenterology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Liver-Targeting Nanoplatforms for the Induction of Immune Tolerance.Nanomaterials (Basel, Switzerland) · 2023Review
- Cell-Mediated Therapies to Facilitate Operational Tolerance in Liver Transplantation.International journal of molecular sciences · 2021Review
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
- Mannosylated liposomes for targeted gene delivery.International journal of nanomedicine · 2012Article
- Dual protective role of HO-1 in transplanted liver grafts: a review of experimental and clinical studies.World journal of gastroenterology · 2011Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop a practical method for isolation, purification and culture of hepatic Kupffer cells (KCs) and to observe their suppressive effects on the proliferation of alloreactive T cells.
methodsPerfusion in situ in vivo combined with density gradient centrifugation was applied in isolation, purification and culture of hepatic KC. The suppression by KCs on the T cell proliferation in mixed lymphocyte reaction (MLR) was observed.
resultsThis method resulted in a satisfactorily high yield of (1.1 +/- 0.2) multiply 10(7) KCs per liver, (93.5% +/- 1.8%) viable cells, over 90% purity and positive for ED-2. After the first 24 h in culture, a great number of KCs which exhibited typical characteristics were observed. Using (3)H-TdR incorporation assay, non-irradiated KCs significantly suppressed allo-MLR. The KCs recovered from accepted liver allografts in groups D and E were more effective in suppressing allo-MLR.
conclusionA standardized procedure for isolation of highly purified rat KCs is proposed and KCs have suppressive effects on the proliferation of alloreactive T cells, especially those derived from accepted liver allografts.
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Registered trials
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