Evidence map›Paper›PMID 17589849›Full record

ArticleCurrent genetics2007

Imaging mycorrhizal fungal transformants that express EGFP during ericoid endosymbiosis.

Elena Martino, Claude Murat, Marta Vallino, Andrea Bena, Silvia Perotto, Pietro Spanu

Abstract read
PubMed Publisher
In one paragraph

Article in Current genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Several Characteristics ofJournal of fungi (Basel, Switzerland) · 2023
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Expanding genomics of mycorrhizal symbiosis.Frontiers in microbiology · 2014
    Review
  11. Review
  12. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Elena MartinoDipartimento di Biologia Vegetale dell'Università di Torino, Centre of Excellence for Plant and Microbial Biosensing (CEBIOVEM) and Istituto per la Protezione delle Piante del CNR, Sezione di Torino, Torino, Italy. elena.martino@unito.it
Claude Murat
Marta Vallino
Andrea Bena
Silvia Perotto
Pietro Spanu
Istituto Nazionale di Fisica Nucleare, Sezione di Torino · ITImperial College London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ericoid endomycorrhizal fungi form intracellular associations with the epidermal root cells of plants belonging to Ericales. In natural environments, these fungi increase the ability of their host plants to colonise soils polluted with toxic metals, although the underlying mechanisms are not clearly understood. Genetic transformation is a powerful tool to study the function of specific genes involved in the interaction of symbiotic fungi with the host plants and with the environment. Here, we investigated the possibility to genetically transform an ericoid endomycorrhizal strain. A metal tolerant mycorrhizal Oidiodendron maius strain isolated from a contaminated area was chosen to develop the transformation system. Two different protocols were used: protoplasts and Agrobacterium-mediated transformation. Stable transformants were obtained with both techniques. They remained competent for mycorrhizal formation and GFP-transformed fungi were visualised in planta. This is the first report of stable transformation of an ericoid endomycorrhizal fungus. The protocol set up could represent a good starting point for the identification of genes important in the ericoid mycorrhiza formation and in the understanding of how this symbiosis is established and functions. The success in the genetic transformation of this strain will allow us to better define its potential use in bioremediation strategies.

Indexed as

Agrobacterium tumefaciensAscomycotaBiodegradation, EnvironmentalGreen Fluorescent ProteinsMetals, HeavyMycorrhizaePlant RootsPlasmidsRecombinant ProteinsSoil MicrobiologySoil PollutantsSymbiosisTransformation, GeneticZincenhanced green fluorescent proteinGreen Fluorescent ProteinsMetals, HeavyRecombinant ProteinsSoil PollutantsZinc

Identifiers

PMID17589849
OpenAlexW2019751971

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.