ArticleThe Journal of biological chemistry2007
Patch clamp and phenotypic analyses of a prokaryotic cyclic nucleotide-gated K+ channel using Escherichia coli as a host.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential.Nature communications · 2020Article
- Functional characterization and optimization of a bacterial cyclic nucleotide-gated channel.The Journal of biological chemistry · 2019Article
- Potassium channel-based optogenetic silencing.Nature communications · 2018Article
- Crystal structures of the TRIC trimeric intracellular cation channel orthologues.Cell research · 2016Article
- The application of theBiotechnology reports (Amsterdam, Netherlands) · 2015Article
- Family of prokaryote cyclic nucleotide-modulated ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Article
- Evolutionary and structural perspectives of plant cyclic nucleotide-gated cation channels.Frontiers in plant science · 2012Article
- A novel manganese efflux system, YebN, is required for virulence by Xanthomonas oryzae pv. oryzae.PloS one · 2011Article
- Structure of the MthK RCK in complex with cadmium.Journal of structural biology · 2010Article
- A novel potassium channel in photosynthetic cyanobacteria.PloS one · 2010Article
- Mg(2+)-dependent gating of bacterial MgtE channel underlies Mg(2+) homeostasis.The EMBO journal · 2009Article
- The desensitization gating of the MthK K+ channel is governed by its cytoplasmic amino terminus.PLoS biology · 2008Article
- Ion channels in microbes.Physiological reviews · 2008Review
- The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution.Structure (London, England : 1993) · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Prokaryotic ion channels have been valuable in providing structural models for understanding ion filtration and channel-gating mechanisms. However, their functional examinations have remained rare and usually been carried out by incorporating purified channel protein into artificial lipid membranes. Here we demonstrate the utilization of Escherichia coli to host the functional analyses by examining a putative cyclic nucleotide-gated K+ channel cloned from Magnetospirillum magnetotacticum, MmaK. When expressed in wild-type E. coli cells, MmaK renders the host sensitive to millimolar concentrations of externally applied K+, indicating MmaK forms a functional K+ conduit in the E. coli membrane in vivo. After enlarging these cells into giant spheroplasts, macro- and microscopic MmaK currents are readily detected in excised E. coli membrane patches by a patch clamp. We show that MmaK is indeed gated by submicromolar cAMP and approximately 10-fold higher concentration of cGMP and manifests as an inwardly rectified, K+-specific current with a 10.8 pS unitary conductance at -100 mV. Additionally, MmaK is inactivated by slightly acidic pH only from the cytoplasmic side. Our in vitro biophysical characterizations of MmaK correlate with its in vivo phenotype in E. coli, implicating its critical role as an intracellular cAMP and pH sensor for modulating bacterial membrane potential. Exemplified by MmaK functional studies, we establish that E. coli and its giant spheroplast provide a convenient and versatile system to express foreign channels for biophysical analyses that can be further dovetailed with microbial genetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.