ArticleBMC cell biology2007
Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells.
Article in BMC cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Pharmacological profile of the neuropeptide S receptor: Dynamic mass redistribution studies.Pharmacology research & perspectives · 2018Article
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- Recent Advances in Biosensing With Photonic Crystal Surfaces: A Review.IEEE sensors journal · 2016Article
- Intensity interrogation near cutoff resonance for label-free cellular profiling.Scientific reports · 2016Article
- Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology.Applied physics letters · 2013Article
- Approaches for probing allosteric interactions at 7 transmembrane spanning receptors.Progress in molecular biology and translational science · 2013Review
- Electrical detection of cancer biomarker using aptamers with nanogap break-junctions.Nanotechnology · 2012Article
- Discovery of 2-(4-methylfuran-2(5H)-ylidene)malononitrile and thieno[3,2-b]thiophene-2-carboxylic acid derivatives as G protein-coupled receptor 35 (GPR35) agonists.Journal of medicinal chemistry · 2011Article
- PROBING CANCER SIGNALING WITH RESONANT WAVEGUIDE GRATING BIOSENSORS.Expert opinion on drug discovery · 2010Article
- Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements.Nature biotechnology · 2010Article
- Non-invasive Optical Biosensor for Probing Cell Signaling.Sensors (Basel, Switzerland) · 2007Review
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2 authors.
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Abstract
backgroundProtease activated receptors (PARs) consist of a family of four G protein-coupled receptors. Many types of cells express several PARs, whose physiological significance is mostly unknown.
resultsHere, we show that non-invasive resonant waveguide grating (RWG) biosensor differentiates signaling of endogenous protease activated receptor subtype 1 (PAR1) and 2 (PAR2) in human epidermoid carcinoma A431 cells. The biosensor directly measures dynamic mass redistribution (DMR) resulted from ligand-induced receptor activation in adherent cells. In A431, both PAR1 and PAR2 agonists, but neither PAR3 nor PAR4 agonists, trigger dose-dependent Ca2+ mobilization as well as Gq-type DMR signals. Both Ca2+ flux and DMR signals display comparable desensitization patterns upon repeated stimulation with different combinations of agonists. However, PAR1 and PAR2 exhibit distinct kinetics of receptor re-sensitization. Furthermore, both trypsin- and thrombin-induced Ca2+ flux signals show almost identical dependence on cell surface cholesterol level, but their corresponding DMR signals present different sensitivities.
conclusionOptical biosensor provides an alternative readout for examining receptor activation under physiologically relevant conditions, and differentiates the signaling of endogenous PAR1 and PAR2 in A431.
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