ArticleProtein science : a publication of the Protein Society2007
Structural and biochemical characterization of a novel Mn2+-dependent phosphodiesterase encoded by the yfcE gene.
Article in Protein science : a publication of the Protein Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Activity and structure ofThe Journal of biological chemistry · 2019Article
- Manganese Is Essential for PlcP Metallophosphoesterase Activity Involved in Lipid Remodeling in Abundant Marine Heterotrophic Bacteria.Applied and environmental microbiology · 2018Article
- Crystal structures of the UDP-diacylglucosamine pyrophosphohydrase LpxH from Pseudomonas aeruginosa.Scientific reports · 2016Article
- Identification and characterization of a novel phosphodiesterase from the metagenome of an Indian coalbed.PloS one · 2015Article
- Crystal structure of the Bacillus subtilis phosphodiesterase PhoD reveals an iron and calcium-containing active site.The Journal of biological chemistry · 2014Article
- The non-catalytic "cap domain" of a mycobacterial metallophosphoesterase regulates its expression and localization in the cell.The Journal of biological chemistry · 2014Article
- Article
- The CphAII protein from Aquifex aeolicus exhibits a metal-dependent phosphodiesterase activity.Extremophiles : life under extreme conditions · 2012Article
- Structural and enzymatic characterization of the streptococcal ATP/diadenosine polyphosphate and phosphodiester hydrolase Spr1479/SapH.The Journal of biological chemistry · 2011Article
- Evolution of the Kdo2-lipid A biosynthesis in bacteria.BMC evolutionary biology · 2010Article
- From complete genome sequence to 'complete' understanding?Trends in biotechnology · 2010Article
- Association between polymorphisms in the metallophosphoesterase (MPPE1) gene and bipolar disorder.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2010Article
- A phosphate-binding histidine of binuclear metallophosphodiesterase enzymes is a determinant of 2',3'-cyclic nucleotide phosphodiesterase activity.The Journal of biological chemistry · 2008Article
- Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase.Nucleic acids research · 2008Article
- Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase.Nucleic acids research · 2007Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Escherichia coli YfcE belongs to a conserved protein family within the calcineurin-like phosphoesterase superfamily (Pfam00149) that is widely distributed in bacteria and archaea. Superfamily members are metallophosphatases that include monoesterases and diesterases involved in a variety of cellular functions. YfcE exhibited catalytic activity against bis-p-nitrophenyl phosphate, a general substrate for phosphodiesterases, and had an absolute requirement for Mn2+. However, no activity was observed with phosphodiesters and over 50 naturally occurring phosphomonoesters. The crystal structure of the YfcE phosphodiesterase has been determined to 2.25 A resolution. YfcE has a beta-sandwich architecture similar to metallophosphatases of common ancestral origin. Unlike its more complex homologs that have added structural elements for regulation and substrate recognition, the relatively small 184-amino-acid protein has retained its ancestral simplicity. The tetrameric protein carries two zinc ions per active site from the E. coli extract that reflect the conserved di-Mn2+ active site geometry. A cocrystallized sulfate inhibitor mimics the binding of phosphate moeities in known ligand/phosphatase complexes. Thus, YfcE has a similar active site and biochemical mechanism as well-characterized superfamily members, while the YfcE phosphodiester-containing substrate is unique.
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