ArticleProtein science : a publication of the Protein Society2007
NMR structure of a KlbA intein precursor from Methanococcus jannaschii.
Article in Protein science : a publication of the Protein Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 47 citations in OpenAlex.
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins.Microorganisms · 2021Article
- Biochemical and Structural Characterization of an Unusual and Naturally Split Class 3 Intein.Chembiochem : a European journal of chemical biology · 2021Article
- Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived fromBiochemistry · 2020Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms.International journal of molecular sciences · 2020Article
- Allosteric Influence of Extremophile Hairpin Motif Mutations on the Protein Splicing Activity of a Hyperthermophilic Intein.Biochemistry · 2020Article
- Mycobacterial DnaB helicase intein as oxidative stress sensor.Nature communications · 2018Article
- Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.Biomolecular NMR assignments · 2015Article
- Structural and dynamical features of inteins and implications on protein splicing.The Journal of biological chemistry · 2014Review
- Protein splicing: how inteins escape from precursor proteins.The Journal of biological chemistry · 2014Review
- Structure-based engineering and comparison of novel split inteins for protein ligation.Molecular bioSystems · 2014Article
- Inteins: Nature's Gift to Protein Chemists.Chemical science · 2014Article
- Recent progress in intein research: from mechanism to directed evolution and applications.Cellular and molecular life sciences : CMLS · 2013Review
- NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein.Journal of molecular biology · 2012Article
- Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii.Biochemistry · 2011Article
- Structural and mutational studies of a hyperthermophilic intein from DNA polymerase II of Pyrococcus abyssi.The Journal of biological chemistry · 2011Article
- 1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein.Biomolecular NMR assignments · 2011Article
- Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Expanding the definition of class 3 inteins and their proposed phage origin.Journal of bacteriology · 2011Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Certain proteins of unicellular organisms are translated as precursor polypeptides containing inteins (intervening proteins), which are domains capable of performing protein splicing. These domains, in conjunction with a single residue following the intein, catalyze their own excision from the surrounding protein (extein) in a multistep reaction involving the cleavage of two intein-extein peptide bonds and the formation of a new peptide bond that ligates the two exteins to yield the mature protein. We report here the solution NMR structure of a 186-residue precursor of the KlbA intein from Methanococcus jannaschii, comprising the intein together with N- and C-extein segments of 7 and 11 residues, respectively. The intein is shown to adopt a single, well-defined globular domain, representing a HINT (Hedgehog/Intein)-type topology. Fourteen beta-strands are arranged in a complex fold that includes four beta-hairpins and an antiparallel beta-ribbon, and there is one alpha-helix, which is packed against the beta-ribbon, and one turn of 3(10)-helix in the loop between the beta-strands 8 and 9. The two extein segments show increased disorder, and form only minimal nonbonding contacts with the intein domain. Structure-based mutation experiments resulted in a proposal for functional roles of individual residues in the intein catalytic mechanism.
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