ArticleRNA (New York, N.Y.)2007
Selection of an improved RNA polymerase ribozyme with superior extension and fidelity.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Exonuclease Ribozyme Encoded in a Circular Genome: On the Initiation of the RNA World.Life (Basel, Switzerland) · 2026Article
- The Emergence of Life in the Light of Evolution.ArXiv · 2026Article
- A small polymerase ribozyme that can synthesize itself and its complementary strand.Science (New York, N.Y.) · 2026Article
- Self-Amplifying RNA: Advantages and Challenges of a Versatile Platform for Vaccine Development.Viruses · 2025Review
- RNA life on the edge of catastrophe.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- RNA-catalyzed evolution of catalytic RNA.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Circular at the very beginning: on the initial genomes in the RNA world.RNA biology · 2024Article
- Origin & influence of autocatalytic reaction networks at the advent of the RNA world.RNA biology · 2024Review
- On the origin of life: an RNA-focused synthesis and narrative.RNA (New York, N.Y.) · 2023Article
- Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit.Biology · 2023Article
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- Pushing the Limits of Nucleic Acid Function.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Review
- Genome Evolution from Random Ligation of RNAs of Autocatalytic Sets.International journal of molecular sciences · 2021Article
- A GTP-synthesizing ribozyme selected by metabolic coupling to an RNA polymerase ribozyme.Science advances · 2021Article
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- Water/Oil Emulsions with Controlled Droplet Sizes forACS omega · 2021Article
- Kinetic Effects of β,γ-Modified Deoxynucleoside 5'-Triphosphate Analogues on RNA-Catalyzed Polymerization of DNA.Biochemistry · 2021Article
- Promiscuous Ribozymes and Their Proposed Role in Prebiotic Evolution.Chemical reviews · 2020Review
- An RNA polymerase ribozyme that synthesizes its own ancestor.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles.Nucleic acids research · 2019Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our current understanding of biology suggests that early life relied predominantly on RNA for catalysis and replication. Here, we report the isolation of an RNA polymerase ribozyme called B6.61 that exhibits superior extension and fidelity relative to its progenitor, the Round-18 polymerase. The B6.61 polymerase was selected from a mutagenized pool containing approximately 9 x 10(14) sequence variants through the use of a novel large-scale in vitro compartmentalization system. B6.61 polymerized all tested primer-template (PT) complexes faster than the Round-18 variant. For one PT, B6.61 exhibited dramatically faster elongation past one full helical turn and incorporated at least 20 nucleotides of sequence, setting a new extension record for an RNA polymerase ribozyme. The increased efficiency of the B6.61 construct was related to improvements in fidelity, with the new variant incorporating less incorrect wobble base pairs than its parent. This new polymerase demonstrates the feasibility of evolving an artificial RNA replicase ribozyme in the foreseeable future.
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