ArticleEukaryotic cell2007
Lia1p, a novel protein required during nuclear differentiation for genome-wide DNA rearrangements in Tetrahymena thermophila.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The Conjusome-A Transient Organelle Linking Genome Rearrangements in the Parental and Developing Macronuclei.Microorganisms · 2023Article
- Roles of Noncoding RNAs in Ciliate Genome Architecture.Journal of molecular biology · 2020Review
- Boundaries of eliminated heterochromatin of Tetrahymena are positioned by the DNA-binding protein Ltl1.Nucleic acids research · 2019Article
- The Co-regulation Data Harvester: automating gene annotation starting from a transcriptome database.SoftwareX · 2017Article
- DRH1, a p68-related RNA helicase gene, is required for chromosome breakage in Tetrahymena.Biology open · 2016Article
- A Parallel G Quadruplex-Binding Protein Regulates the Boundaries of DNA Elimination Events of Tetrahymena thermophila.PLoS genetics · 2016Article
- Chromodomain protein Tcd1 is required for macronuclear genome rearrangement and repair in Tetrahymena.Scientific reports · 2015Article
- Tetrahymena Pot2 is a developmentally regulated paralog of Pot1 that localizes to chromosome breakage sites but not to telomeres.Eukaryotic cell · 2014Article
- LIA4 encodes a chromoshadow domain protein required for genomewide DNA rearrangements in Tetrahymena thermophila.Eukaryotic cell · 2014Article
- Mutations in Pdd1 reveal distinct requirements for its chromodomain and chromoshadow domain in directing histone methylation and heterochromatin elimination.Eukaryotic cell · 2014Article
- Review
- Article
- An essential role for the DNA breakage-repair protein Ku80 in programmed DNA rearrangements in Tetrahymena thermophila.Molecular biology of the cell · 2012Article
- Tetrahymena thermophila JMJD3 homolog regulates H3K27 methylation and nuclear differentiation.Eukaryotic cell · 2012Article
- Genome-Scale Analysis of Programmed DNA Elimination Sites in Tetrahymena thermophila.G3 (Bethesda, Md.) · 2011Article
- Keeping the soma free of transposons: programmed DNA elimination in ciliates.The Journal of biological chemistry · 2011Review
- Programmed DNA elimination in Tetrahymena: a small RNA-mediated genome surveillance mechanism.Advances in experimental medicine and biology · 2011Review
- The conjugation-specific Die5 protein is required for development of the somatic nucleus in both Paramecium and Tetrahymena.Eukaryotic cell · 2010Article
- A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila.Molecular biology of the cell · 2010Article
- Microarray analyses of gene expression during the Tetrahymena thermophila life cycle.PloS one · 2009Article
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2 authors.
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Abstract
Extensive genome-wide rearrangements occur during somatic macronuclear development in Tetrahymena thermophila. These events are guided by RNA interference-directed chromatin modification including histone H3 lysine 9 methylation, which marks specific germ line-limited internal eliminated sequences (IESs) for excision. Several genes putatively involved in these developmental genome rearrangements were identified based on their proteins' localization to differentiating somatic nuclei, and here we demonstrate that one, LIA1, encodes a novel protein that is an essential component of the genome rearrangement machinery. A green fluorescent protein-Lia1 fusion protein exhibited dynamic nuclear localization during development that has striking similarity to that of the dual chromodomain-containing DNA rearrangement protein, Pdd1p. Coimmunoprecipitation experiments showed that Lia1p associates with Pdd1p and IES chromatin during macronuclear development. Cell lines in which we disrupted both the germ line and somatic copies of LIA1 (DeltaLIA1) grew normally but were unable to generate viable progeny, arresting late in development just prior to returning to vegetative growth. These mutant lines failed to properly form Pdd1p-containing nuclear structures and eliminate IESs despite showing normal levels of H3K9 methylation. These data indicate that Lia1p is required late in conjugation for the reorganization of the Tetrahymena genome.
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