ArticleEukaryotic cell2007
EstB-mediated hydrolysis of the siderophore triacetylfusarinine C optimizes iron uptake of Aspergillus fumigatus.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 77 citations in OpenAlex.
- Siderophore Activity of Partially Acetylated Fusarinines from the Sponge-Derived FungusJournal of natural products · 2025Article
- Review
- Discovery of Peptidic Siderophore Degradation by Screening Natural Product Profiles in Marine-Derived Bacterial Mono- and Cocultures.Biochemistry · 2025Article
- Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi.Journal of microbiology and biotechnology · 2024Review
- The Transcription Factors AcuK and AcuM Influence Siderophore Biosynthesis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Inactivation of siderophore iron-chelating moieties by the fungal wheat root symbiont Pyrenophora biseptata.Environmental microbiology reports · 2024Article
- Regulatory and pathogenic mechanisms in response to iron deficiency and excess in fungi.Microbial biotechnology · 2023Review
- Fungal siderophore metabolism with a focus on Aspergillus fumigatus: impact on biotic interactions and potential translational applications.Essays in biochemistry · 2023Article
- Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Synergistic Antifungal Effect of a Combination of Iron Deficiency and Calcium Supplementation.Microbiology spectrum · 2022Article
- Antibacterial diphenyl ether production induced by co-culture of Aspergillus nidulans and Aspergillus fumigatus.Applied microbiology and biotechnology · 2022Article
- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- Nitrogen, Iron and Zinc Acquisition: Key Nutrients toJournal of fungi (Basel, Switzerland) · 2021Review
- Identification of N,N',N″-triacetylfusarinine C as a key metabolite for root rot disease virulence in American ginseng.Journal of ginseng research · 2021Article
- Siderophore-Based Molecular Imaging of Fungal and Bacterial Infections-Current Status and Future Perspectives.Journal of fungi (Basel, Switzerland) · 2020Review
- Metabolomic Profiling of Fungal Pathogens Responsible for Root Rot in American Ginseng.Metabolites · 2020Article
- Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex ofGenetics · 2019Article
- An insight into the iron acquisition and homeostasis in Aureobasidium melanogenum HN6.2 strain through genome mining and transcriptome analysis.Functional & integrative genomics · 2019Article
- Host-Pathogen Interactions Mediated by MDR Transporters in Fungi: As Pleiotropic as it Gets!Genes · 2018Review
- Genomic Organization and Expression of Iron Metabolism Genes in the Emerging Pathogenic MoldFrontiers in microbiology · 2018Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
Aspergillus fumigatus excretes the fusarinine-type siderophore desferri-triacetylfusarinine C (DF-TafC) to mobilize iron. DF-TafC is a cyclic peptide consisting of three N(5)-cis-anhydromevalonyl-N(5)-hydroxy-N(2)-acetyl-l-ornithine residues linked by ester bonds; these linkages are in contrast to peptide linkages found for ferrichrome-type siderophores. Subsequent to the binding of iron and uptake, triacetylfusarinine C (TafC) is hydrolyzed, the cleavage products are excreted, and the iron is transferred to the metabolism or to the intracellular siderophore desferri-ferricrocin (DF-FC) for iron storage. Here we report the identification and characterization of the TafC esterase EstB, the first eukaryotic siderophore-degrading enzyme to be characterized at the molecular level. The encoding gene, estB, was found to be located in an iron-regulated gene cluster, indicating a role in iron metabolism. Deletion of estB in A. fumigatus eliminated TafC esterase activity of cellular extracts and caused increased intracellular accumulation of TafC and TafC hydrolysis products in vivo. Escherichia coli-expressed EstB displayed specific TafC esterase activity but did not hydrolyze fusarinine C, which has the same core structure as TafC but lacks three N(2)-acetyl residues. Localization of EstB via enhanced green fluorescent protein tagging suggested that TafC hydrolysis takes place in the cytoplasm. EstB abrogation reduced the intracellular transfer rate of iron from TafC to DF-FC and delayed iron sensing. Furthermore, EstB deficiency caused a decreased radial growth rate under iron-depleted but not iron-replete conditions. Taken together, these data suggest that EstB-mediated TafC hydrolysis optimizes but is not essential for TafC-mediated iron uptake in A. fumigatus.
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