ArticleApplied and environmental microbiology2007
Development of a swine-specific fecal pollution marker based on host differences in methanogen mcrA genes.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Archaeome structure and function of the intestinal tract in healthy and H1N1 infected swine.Frontiers in microbiology · 2023Article
- Source tracking swine fecal waste in surface water proximal to swine concentrated animal feeding operations.The Science of the total environment · 2015Article
- Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR.MicrobiologyOpen · 2014Article
- Microbial trophic interactions and mcrA gene expression in monitoring of anaerobic digesters.Frontiers in microbiology · 2014Review
- "Methanoplasmatales," Thermoplasmatales-related archaea in termite guts and other environments, are the seventh order of methanogens.Applied and environmental microbiology · 2012Article
- Oligonucleotide primers, probes and molecular methods for the environmental monitoring of methanogenic archaea.Microbial biotechnology · 2011Review
- Comparative fecal metagenomics unveils unique functional capacity of the swine gut.BMC microbiology · 2011Article
- Molecular indicators used in the development of predictive models for microbial source tracking.Applied and environmental microbiology · 2010Article
- Evaluation of Lactobacillus sobrius/L. amylovorus as a new microbial marker of pig manure.Applied and environmental microbiology · 2010Article
- Evaluation of swine-specific PCR assays used for fecal source tracking and analysis of molecular diversity of swine-specific "bacteroidales" populations.Applied and environmental microbiology · 2009Article
- Microbial diversity and host-specific sequences of Canada goose feces.Applied and environmental microbiology · 2009Article
- Pig manure contamination marker selection based on the influence of biological treatment on the dominant fecal microbial groups.Applied and environmental microbiology · 2009Article
- Estimation of pig fecal contamination in a river catchment by real-time PCR using two pig-specific Bacteroidales 16S rRNA genetic markers.Applied and environmental microbiology · 2009Article
- Methanobrevibacter ruminantium as an indicator of domesticated-ruminant fecal pollution in surface waters.Applied and environmental microbiology · 2007Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The goal of this study was to evaluate methanogen diversity in animal hosts to develop a swine-specific archaeal molecular marker for fecal source tracking in surface waters. Phylogenetic analysis of swine mcrA sequences compared to mcrA sequences from the feces of five animals (cow, deer, sheep, horse, and chicken) and sewage showed four distinct swine clusters, with three swine-specific clades. From this analysis, six sequences were chosen for molecular marker development and initial testing. Only one mcrA sequence (P23-2) showed specificity for swine and therefore was used for environmental testing. PCR primers for the P23-2 clone mcrA sequence were developed and evaluated for swine specificity. The P23-2 primers amplified products in P23-2 plasmid DNA (100%), pig feces (84%), and swine waste lagoon surface water samples (100%) but did not amplify a product in 47 bacterial and archaeal stock cultures and 477 environmental bacterial isolates and sewage and water samples from a bovine waste lagoon and a polluted creek. Amplification was observed in only one sheep sample out of 260 human and nonswine animal fecal samples. Sequencing of PCR products from pig feces demonstrated 100% similarity to pig mcrA sequence from clone P23-2. The minimal amount of DNA required for the detection was 1 pg for P23-2 plasmid, 1 ng for pig feces, 50 ng for swine waste lagoon surface water, 1 ng for sow waste influent, and 10 ng for lagoon sludge samples. Lower detection limits of 10(-6) g of wet pig feces in 500 ml of phosphate-buffered saline and 10(-4) g of lagoon waste in estuarine water were established for the P23-2 marker. This study was the first to utilize methanogens for the development of a swine-specific fecal contamination marker.
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