ArticleJournal of bacteriology2007
GroEL plays a central role in stress-induced negative regulation of bacterial conjugation by promoting proteolytic degradation of the activator protein TraJ.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Regulation of RNase PH during nutrient deprivation: the role of proteases, GroEL, and RNase II.mBio · 2026Article
- Complex compositional and metabolic response of river sediment microbiomes to multiple anthropogenic stressors.ISME communications · 2026Article
- Article
- Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level.Genes · 2020Review
- Protein Dynamics in F-like Bacterial Conjugation.Biomedicines · 2020Review
- Successful Host Adaptation of IncK2 Plasmids.Frontiers in microbiology · 2019Article
- Natural and Artificial Strategies To Control the Conjugative Transmission of Plasmids.Microbiology spectrum · 2018Review
- Article
- Crystal structures of the F and pSLT plasmid TraJ N-terminal regions reveal similar homodimeric PAS folds with functional interchangeability.Biochemistry · 2014Article
- Social behavior and decision making in bacterial conjugation.Frontiers in cellular and infection microbiology · 2014Review
- Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ.Journal of bacteriology · 2012Article
- Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control.Microbial cell factories · 2011Article
- Side effects of chaperone gene co-expression in recombinant protein production.Microbial cell factories · 2010Review
- Regulation of bacterial conjugation in microaerobiosis by host-encoded functions ArcAB and sdhABCD.Genetics · 2010Article
- Biological diversity of prokaryotic type IV secretion systems.Microbiology and molecular biology reviews : MMBR · 2009Review
- Functional definition and global regulation of Zur, a zinc uptake regulator in a Streptococcus suis serotype 2 strain causing streptococcal toxic shock syndrome.Journal of bacteriology · 2008Article
- Spatial structure and nutrients promote invasion of IncP-1 plasmids in bacterial populations.The ISME journal · 2008Article
- Conjugal transfer of the Salmonella enterica virulence plasmid in the mouse intestine.Journal of bacteriology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of DNA transfer genes is a prerequisite for conjugative DNA transfer of F-like plasmids. Transfer gene expression is sensed by the donor cell and is regulated by a complex network of plasmid- and host-encoded factors. In this study we analyzed the effect of induction of the heat shock regulon on transfer gene expression and DNA transfer in Escherichia coli. Raising the growth temperature from 22 degrees C to 43 degrees C transiently reduced transfer gene expression to undetectable levels and reduced conjugative transfer by 2 to 3 orders of magnitude. In contrast, when host cells carried the temperature-sensitive groEL44 allele, heat shock-mediated repression was alleviated. These data implied that the chaperonin GroEL was involved in negative regulation after heat shock. Investigation of the role of GroEL in this regulatory process revealed that, in groEL(Ts) cells, TraJ, the plasmid-encoded master activator of type IV secretion (T4S) system genes, was less susceptible to proteolysis and had a prolonged half-life compared to isogenic wild-type E. coli cells. This result suggested a direct role for GroEL in proteolysis of TraJ, down-regulation of T4S system gene expression, and conjugation after heat shock. Strong support for this novel role for GroEL in regulation of bacterial conjugation was the finding that GroEL specifically interacted with TraJ in vivo. Our results further suggested that in wild-type cells this interaction was followed by rapid degradation of TraJ whereas in groEL(Ts) cells TraJ remained trapped in the temperature-sensitive GroEL protein and thus was not amenable to proteolysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.