ReviewMethods in cell biology2007
Laser microsurgery in the GFP era: a cell biologist's perspective.
Review in Methods in cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Mechanical Coupling With the Nuclear Envelope Shapes the Schizosaccharomyces pombe Mitotic Spindle.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond.ACS photonics · 2024Review
- Coupling of microtubule bundles isolates them from local disruptions to set the structural stability of the anaphase spindle.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine.Micromachines · 2021Article
- Chemical tools for dissecting cell division.Nature chemical biology · 2021Review
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- Laser Scissors and Tweezers to Study Chromosomes: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Force balances between interphase centrosomes as revealed by laser ablation.Molecular biology of the cell · 2019Article
- SPR2 protects minus ends to promote severing and reorientation of plant cortical microtubule arrays.The Journal of cell biology · 2018Article
- Optogenetic control of kinetochore function.Nature chemical biology · 2017Article
- Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.Current biology : CB · 2017Article
- Microfluidic guillotine for single-cell wound repair studies.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Laser-based technique for controlled damage of mesenchymal cell spheroids: a first step in studying reparation in vitro.Biology open · 2016Article
- Excess centrosomes disrupt endothelial cell migration via centrosome scattering.The Journal of cell biology · 2014Article
- Direct kinetochore-spindle pole connections are not required for chromosome segregation.The Journal of cell biology · 2014Article
- Golgi as an MTOC: making microtubules for its own good.Histochemistry and cell biology · 2013Review
- Cell signaling experiments driven by optical manipulation.International journal of molecular sciences · 2013Review
- Predictive-focus illumination for reducing photodamage in live-cell microscopy.Journal of microscopy · 2012Article
- Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance.Molecular biology of the cell · 2012Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Modern biology is based largely on a reductionistic "dissection" approach-most cell biologists try to determine how complex biological systems work by removing their individual parts and studying the effects of this removal on the system. A variety of enzymatic and mechanical methods have been developed to dissect large cell assemblies like tissues and organs. Further, individual proteins can be inactivated or removed within a cell by genetic manipulations (e.g., RNAi or gene knockouts). However, there is a growing demand for tools that allow intracellular manipulations at the level of individual organelles. Laser microsurgery is ideally suited for this purpose and the popularity of this approach is on the rise among cell biologists. In this chapter, we review some of the applications for laser microsurgery at the subcellular level and describe practical requirements for laser microsurgery instrumentation demanded in the field. We also outline a relatively inexpensive but versatile laser microsurgery workstation that is being used in our laboratory. Our major thesis is that the limitations of the technology are no longer at the level of the laser, microscope, or software, but instead only in defining creative questions and in visualizing the target to be destroyed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.