ArticleNucleic acids research2007
Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Mechanisms and Strategies for Enhancing DNA Nuclear Entry in Gene Delivery.Cellular and molecular bioengineering · 2026Article
- Mapping cellular processes that determine delivery of plasmid DNA to the nucleus: application in Chinese hamster ovary and human embryonic kidney cells to enhance protein production.Frontiers in bioengineering and biotechnology · 2025Article
- New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment.International journal of molecular sciences · 2022Review
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
- Facilitating plasmid nuclear delivery by interfering with the selective nuclear pore barrier.Bioengineering & translational medicine · 2019Article
- Enhancing Electrotransfection Efficiency through Improvement in Nuclear Entry of Plasmid DNA.Molecular therapy. Nucleic acids · 2018Article
- The Length of Hydrophobic Chain in Amphiphilic Polypeptides Regulates the Efficiency of Gene Delivery.Polymers · 2018Article
- Non-viral Delivery of Nucleic Acids: Insight Into Mechanisms of Overcoming Intracellular Barriers.Frontiers in pharmacology · 2018Review
- Cytoplasmic transport and nuclear import of plasmid DNA.Bioscience reports · 2017Review
- Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells.Biochemistry and biophysics reports · 2016Article
- Integrated Electrowetting Nanoinjector for Single Cell Transfection.Scientific reports · 2016Article
- Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Clathrin inhibitor Pitstop-2 disrupts the nuclear pore complex permeability barrier.Scientific reports · 2015Article
- Nanotherapy for Cancer: Targeting and Multifunctionality in the Future of Cancer Therapies.ACS biomaterials science & engineering · 2015Review
- In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation.Nature protocols · 2015Article
- Challenges associated with Penetration of Nanoparticles across Cell and Tissue Barriers: A Review of Current Status and Future Prospects.Nano today · 2014Article
- In vivo reprogramming of circuit connectivity in postmitotic neocortical neurons.Nature neuroscience · 2013Article
- Amiloride enhances antigen specific CTL by faciliting HBV DNA vaccine entry into cells.PloS one · 2012Article
- Octa-functional PLGA nanoparticles for targeted and efficient siRNA delivery to tumors.Biomaterials · 2012Article
- Gene transfer: how can the biological barriers be overcome?The Journal of membrane biology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the major obstacles in non-viral gene transfer is the nuclear membrane. Attempts to improve the transport of DNA to the nucleus through the use of nuclear localization signals or importin-beta have achieved limited success. It has been proposed that the nuclear pore complexes (NPCs) through which nucleocytoplasmic transport occurs are filled with a hydrophobic phase through which hydrophobic importins can dissolve. Therefore, considering the hydrophobic nature of the NPC channel, we evaluated whether a non-selective gating of nuclear pores by trans-cyclohexane-1,2-diol (TCHD), an amphipathic alcohol that reversibly collapses the permeability barrier of the NPCs, could be obtained and used as an alternative method to facilitate nuclear entry of plasmid DNA. Our data demonstrate for the first time that TCHD makes the nucleus permeable for both high molecular weight dextrans and plasmid DNA (pDNA) at non-toxic concentrations. Furthermore, in line with these observations, TCHD enhanced the transfection efficacy of both naked DNA and lipoplexes. In conclusion, based on the proposed structure of NPCs we succeeded to temporarily open the NPCs for macromolecules as large as pDNAs and demonstrated that this can significantly enhance non-viral gene delivery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.