ArticleBMC genomics2007
New criteria for selecting the origin of DNA replication in Wolbachia and closely related bacteria.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 44 citations in OpenAlex.
- Within-Host Environmental Heterogeneity Is Associated With Phenotypic but Not Genomic Diversity in Wolbachia Endosymbionts.Environmental microbiology reports · 2026Article
- Antibiotic agrochemical treatment reduces endosymbiont infections and alters population dynamics in leafminers, thrips, and parasitoid wasps.Frontiers in microbiology · 2025Article
- Two Complete Genomes of Male-Killing Wolbachia Infecting Ostrinia Moth Species Illuminate Their Evolutionary Dynamics and Association with Hosts.Microbial ecology · 2023Article
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- One to host them all: genomics of the diverse bacterial endosymbionts of the spiderMicrobial genomics · 2023Article
- Phylogenomic analysis of Wolbachia genomes from the Darwin Tree of Life biodiversity genomics project.PLoS biology · 2023Article
- Widespread phages of endosymbionts: Phage WO genomics and the proposed taxonomic classification of Symbioviridae.PLoS genetics · 2022Article
- Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis.BMC genomics · 2021Article
- Diminutive, degraded but dissimilar:Microbial genomics · 2020Article
- Complete Genome Sequence ofMicrobiology resource announcements · 2020Article
- Complete Genome Sequence of the Wolbachia wAlbB Endosymbiont of Aedes albopictus.Genome biology and evolution · 2019Article
- A bioinformatics approach to identifyingPeerJ · 2018Article
- Analysis of complete genome sequence and major surface antigens of Neorickettsia helminthoeca, causative agent of salmon poisoning disease.Microbial biotechnology · 2017Article
- Supergroup C Wolbachia, mutualist symbionts of filarial nematodes, have a distinct genome structure.Open biology · 2015Article
- Comparative analysis of wolbachia genomes reveals streamlining and divergence of minimalist two-component systems.G3 (Bethesda, Md.) · 2015Article
- Maintenance of chromosome structure in Pseudomonas aeruginosa.FEMS microbiology letters · 2014Review
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- Improving Sterile Insect Technique (SIT) for tsetse flies through research on their symbionts and pathogens.Journal of invertebrate pathology · 2013Review
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- Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector.Genetics and molecular biology · 2011Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe annotated genomes of two closely related strains of the intracellular bacterium Wolbachia pipientis have been reported without the identifications of the putative origin of replication (ori). Identifying the ori of these bacteria and related alpha-Proteobacteria as well as their patterns of sequence evolution will aid studies of cell replication and cell density, as well as the potential genetic manipulation of these widespread intracellular bacteria.
resultsUsing features that have been previously experimentally verified in the alpha-Proteobacterium Caulobacter crescentus, the origin of DNA replication (ori) regions were identified in silico for Wolbachia strains and eleven other related bacteria belonging to Ehrlichia, Anaplasma, and Rickettsia genera. These features include DnaA-, CtrA- and IHF-binding sites as well as the flanking genes in C. crescentus. The Wolbachia ori boundary genes were found to be hemE and COG1253 protein (CBS domain protein). Comparisons of the putative ori region among related Wolbachia strains showed higher conservation of bases within binding sites.
conclusionThe sequences of the ori regions described here are only similar among closely related bacteria while fundamental characteristics like presence of DnaA and IHF binding sites as well as the boundary genes are more widely conserved. The relative paucity of CtrA binding sites in the ori regions, as well as the absence of key enzymes associated with DNA replication in the respective genomes, suggest that several of these obligate intracellular bacteria may have altered replication mechanisms. Based on these analyses, criteria are set forth for identifying the ori region in genome sequencing projects.
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