ArticleBiochemistry2007
Structural characterization of human RPA sequential binding to single-stranded DNA using ssDNA as a molecular ruler.
Article in Biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Replication of [AT/TA]Biochemistry · 2024Article
- Rapid Long-distance Migration of RPA on Single Stranded DNA Occurs Through Intersegmental Transfer Utilizing Multivalent Interactions.Journal of molecular biology · 2024Article
- Hydrogen-deuterium exchange reveals a dynamic DNA-binding map of replication protein A.Nucleic acids research · 2021Article
- SV40 T antigen interactions with ssDNA and replication protein A: a regulatory role of T antigen monomers in lagging strand DNA replication.Nucleic acids research · 2020Article
- Roles of OB-Fold Proteins in Replication Stress.Frontiers in cell and developmental biology · 2020Review
- Secondary structure forming sequences drive SD-MMEJ repair of DNA double-strand breaks.Nucleic acids research · 2017Article
- 5' to 3' Unfolding Directionality of DNA Secondary Structures by Replication Protein A: G-QUADRUPLEXES AND DUPLEXES.The Journal of biological chemistry · 2016Article
- A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution.Nucleic acids research · 2016Article
- Dynamic binding of replication protein a is required for DNA repair.Nucleic acids research · 2016Article
- Molecular Evolution and Functional Diversification of Replication Protein A1 in Plants.Frontiers in plant science · 2016Article
- Mechanochemical regulations of RPA's binding to ssDNA.Scientific reports · 2015Article
- HCoDES reveals chromosomal DNA end structures with single-nucleotide resolution.Molecular cell · 2014Article
- G-quadruplex formation in telomeres enhances POT1/TPP1 protection against RPA binding.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RPA-mediated unfolding of systematically varying G-quadruplex structures.Biophysical journal · 2013Article
- A new structural framework for integrating replication protein A into DNA processing machinery.Nucleic acids research · 2013Article
- Structure and conformational change of a replication protein A heterotrimer bound to ssDNA.Genes & development · 2012Article
- Theoretical prediction of the binding free energy for mutants of replication protein A.Journal of molecular modeling · 2012Article
- Accurate prediction of the binding free energy and analysis of the mechanism of the interaction of replication protein A (RPA) with ssDNA.Journal of molecular modeling · 2012Article
- Replication protein A unfolds G-quadruplex structures with varying degrees of efficiency.The journal of physical chemistry. B · 2012Article
- Repair-specific functions of replication protein A.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Human replication protein A (RPA), a heterotrimer composed of RPA70, RPA32, and RPA14 subunits, contains four single-stranded DNA (ssDNA) binding domains (DBD): DBD-A, DBD-B, and DBD-C in RPA70 and DBD-D in RPA32. Although crystallographic or NMR structures of these DBDs and a trimerization core have been determined, the structure of the full length of RPA or the RPA-ssDNA complex remains unknown. In this article, we have examined the structural features of RPA interaction with ssDNA by fluorescence spectroscopy. Using a set of oligonucleotides (dT) with varying lengths as a molecular ruler and also as the substrate, we have determined at single-nucleotide resolution the relative positions of the ssDNA with interacting intrinsic tryptophans of RPA. Our results revealed that Trp528 in DBD-C and Trp107 in DBD-D contact ssDNA at the 16th and 24th nucleotides (nt) from the 5'-end of the substrate, respectively. Evaluation of the relative spatial arrangement of RPA domains in the RPA-ssDNA complex suggested that DBD-B and DBD-C are spaced by about 4 nt ( approximately 19 A) apart, whereas DBD-C and DBD-D are spaced by about 7 nt ( approximately 34 A). On the basis of these geometric constraints, a global structure model for the binding of the major RPA DBDs to ssDNA was proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.