ArticleCurrent biology : CB2007
Cycling of CRYPTOCHROME proteins is not necessary for circadian-clock function in mammalian fibroblasts.
Article in Current biology : CB, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 66 citations in OpenAlex.
- Circadian rhythms in stem cells and their therapeutic potential.Stem cell research & therapy · 2025Review
- PERspectives on circadian cell biology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Development of compounds for targeted degradation of mammalian cryptochrome proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- The circadian clock in the choroid plexus drives rhythms in multiple cellular processes under the control of the suprachiasmatic nucleus.Fluids and barriers of the CNS · 2024Article
- Article
- Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations.Scientific reports · 2022Article
- Cryptochrome 1 as a state variable of the circadian clockwork of the suprachiasmatic nucleus: Evidence from translational switching.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- CRYPTOCHROMES confer robustness, not rhythmicity, to circadian timekeeping.The EMBO journal · 2021Article
- Non-transcriptional processes in circadian rhythm generation.Current opinion in physiology · 2018Review
- Design Principles of Phosphorylation-Dependent Timekeeping in Eukaryotic Circadian Clocks.Cold Spring Harbor perspectives in biology · 2018Review
- Transcriptome profiling of PeCRY1 transgenic Populus tomentosa.Genes & genomics · 2018Article
- Evaluation of circadian phenotypes utilizing fibroblasts from patients with circadian rhythm sleep disorders.Translational psychiatry · 2017Article
- Life in a dark biosphere: a review of circadian physiology in "arrhythmic" environments.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Review
- Protein sequestration versus Hill-type repression in circadian clock models.IET systems biology · 2016Review
- Rhythmic expression of cryptochrome induces the circadian clock of arrhythmic suprachiasmatic nuclei through arginine vasopressin signaling.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Reciprocal Control of the Circadian Clock and Cellular Redox State - a Critical Appraisal.Molecules and cells · 2016Review
- Distinct and separable roles for endogenous CRY1 and CRY2 within the circadian molecular clockwork of the suprachiasmatic nucleus, as revealed by the Fbxl3(Afh) mutation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Development of transgenic minipigs with expression of antimorphic human cryptochrome 1.PloS one · 2013Article
- Does the precision of a biological clock depend upon its period? Effects of the duper and tau mutations in Syrian hamsters.PloS one · 2012Article
- Epigenetic Control of Circadian Clock Operation during Development.Genetics research international · 2012Article
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5 authors at 1 institution in 1 country.
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Abstract
backgroundAn interlocked transcriptional-translational feedback loop (TTFL) is thought to generate the mammalian circadian clockwork in both the central pacemaker residing in the hypothalamic suprachiasmatic nuclei and in peripheral tissues. The core circadian genes, including Period1 and Period2 (Per1 and Per2), Cryptochrome1 and Cryptochrome2 (Cry1 and Cry2), Bmal1, and Clock are indispensable components of this biological clockwork. The cycling of the PER and CRY clock proteins has been thought to be necessary to keep the mammalian clock ticking.
resultsWe provide a novel cell-permeant protein approach for manipulating cryptochrome protein levels to evaluate the current transcription and translation feedback model of the circadian clockwork. Cell-permeant cryptochrome proteins appear to be functional on the basis of several criteria, including the abilities to (1) rescue circadian properties in Cry1(-/-)Cry2(-/-) mouse fibroblasts, (2) act as transcriptional repressors, and (3) phase shift the circadian oscillator in Rat-1 fibroblasts. By using cell-permeant cryptochrome proteins, we demonstrate that cycling of CRY1, CRY2, and BMAL1 is not necessary for circadian-clock function in fibroblasts.
conclusionsThese results are not supportive of the current version of the transcription and translation feedback-loop model of the mammalian clock mechanism, in which cycling of the essential clock proteins CRY1 and CRY2 is thought to be necessary.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.