ArticleAmerican journal of physiology. Renal physiology2007
Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells.
Article in American journal of physiology. Renal physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- The Physiological Function and Potential Role of the Ubiquitous NaInternational journal of molecular sciences · 2022Review
- The HVCN1 voltage-gated proton channel contributes to pH regulation in canine ventricular myocytes.The Journal of physiology · 2022Article
- The Role of Plasma Membrane Sodium/Hydrogen Exchangers in Gastrointestinal Functions: Proliferation and Differentiation, Fluid/Electrolyte Transport and Barrier Integrity.Frontiers in physiology · 2022Review
- Roles of Endomembrane Alkali Cation/Proton Exchangers in Synaptic Function and Neurodevelopmental Disorders.Frontiers in physiology · 2022Review
- Novel Treatments from Inhibition of the Intestinal Sodium-Hydrogen Exchanger 3.International journal of nephrology and renovascular disease · 2021Review
- Essential function of NHE8 in mouse retina demonstrated by AAV-mediated CRISPR/Cas9 knockdown.Experimental eye research · 2018Article
- Developmental changes in renal tubular transport-an overview.Pediatric nephrology (Berlin, Germany) · 2015Review
- Traditional and emerging roles for the SLC9 Na+/H+ exchangers.Pflugers Archiv : European journal of physiology · 2014Review
- The erythropoietin receptor is a downstream effector of Klotho-induced cytoprotection.Kidney international · 2013Article
- Proximal tubular NHEs: sodium, protons and calcium?American journal of physiology. Renal physiology · 2013Review
- Glucocorticoids Reduce Renal NHE8 Expression.Physiological reports · 2013Article
- Acid increases NHE8 surface expression and activity in NRK cells.American journal of physiology. Renal physiology · 2012Article
- Nucleotide sequence of the Na+/H+ exchanger-8 in patients with congenital sodium diarrhea.Journal of pediatric gastroenterology and nutrition · 2011Article
- Silencing of sodium-hydrogen exchanger 1 attenuates the proliferation, hypertrophy, and migration of pulmonary artery smooth muscle cells via E2F1.American journal of respiratory cell and molecular biology · 2011Article
- Effect of metabolic acidosis on neonatal proximal tubule acidification.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- The sodium/proton exchanger NHE8 regulates late endosomal morphology and function.Molecular biology of the cell · 2010Article
- Mechanisms of the regulation of the intestinal Na+/H+ exchanger NHE3.Journal of biomedicine & biotechnology · 2010Review
- The regulation of proximal tubular salt transport in hypertension: an update.Current opinion in nephrology and hypertension · 2009Review
- Luminal Na(+)/H (+) exchange in the proximal tubule.Pflugers Archiv : European journal of physiology · 2009Review
- NHE8 is an intracellular cation/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti.American journal of physiology. Renal physiology · 2009Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
NHE8 is expressed in the apical membrane of the proximal tubule and is predicted to be a Na+/H+ exchanger on the basis of its primary amino acid sequence. Functional characterization of native NHE8 in mammalian cells has not been possible to date. We screened a number of polarized renal cell lines for the plasma membrane Na+/H+ exchangers (NHE1, 2, 3, 4, and 8) and found only NHE1 and NHE8 transcripts in NRK cells by RT-PCR. NHE8 protein is expressed in the apical membrane of NRK cells as demonstrated by immunoblots, confocal fluorescent immunocytochemistry, and immunoelectron microscopy. NHE1, on the other hand, is expressed primarily in the basolateral membrane. Bilateral perfusion of NRK cells grown on permeable supports shows Na+/H+ exchange activity on both the apical and basolateral membranes. NHE8-specific small interfering RNA knocks down NHE8 protein expression but does not affect NHE1 protein levels. Knockdown of NHE8 protein is accompanied by a commensurate reduction in apical NHE activity, without altered basolateral NHE activity. Conversely, transfection of NHE1-specific small interfering RNA knocks down NHE1 protein expression without affecting NHE8 protein levels and reduces basolateral NHE activity without affecting apical NHE activity. NHE8 is the only apical membrane Na+/H+ exchanger in NRK cells. NHE8 activity is Na+ dependent, displaying a cooperative sigmoidal relationship, and is highly sensitive to 5-(N-ethyl-n-isopropyl)-amiloride (EIPA). NRK cells provide a useful system where NHE8 can be studied in its native environment.
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