ArticleBiochemistry2007
Modification by acrolein, a component of tobacco smoke and age-related oxidative stress, mediates functional impairment of human apolipoprotein E.
Article in Biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Potential Adverse Public Health Effects Afforded by the Ingestion of Dietary Lipid Oxidation Product Toxins: Significance of Fried Food Sources.Nutrients · 2020Pooled it
- The Effect of Organic Matter from Sewage Sludge as an Interfacial Layer on the Surface of Nano-Al and Fluoride.Molecules (Basel, Switzerland) · 2023Article
- The Impact of High-Intensity Ultrasound-Assisted Extraction on the Structural and Functional Properties of Hempseed Protein Isolate (HPI).Foods (Basel, Switzerland) · 2023Article
- Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats-A Comparison with Mesna.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Lipoproteins as targets and markers of lipoxidation.Redox biology · 2019Review
- Mechanisms Underlying Acrolein-Mediated Inhibition of Chromatin Assembly.Molecular and cellular biology · 2016Article
- Article
- Acrolein modification impairs key functional features of rat apolipoprotein E: identification of modified sites by mass spectrometry.Biochemistry · 2014Article
- Interaction of aldehydes derived from lipid peroxidation and membrane proteins.Frontiers in physiology · 2013Review
- Biochemical and biophysical characterization of recombinant rat apolipoprotein E: similarities to human apolipoprotein E3.Archives of biochemistry and biophysics · 2013Article
- The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats.Journal of cellular and molecular medicine · 2011Article
- Apolipoprotein E LDL receptor-binding domain-containing high-density lipoprotein: a nanovehicle to transport curcumin, an antioxidant and anti-amyloid bioflavonoid.Biochimica et biophysica acta · 2011Article
- Full-length apolipoprotein E protects against the neurotoxicity of an apoE-related peptide.Brain research · 2010Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Oxidative damage to proteins such as apolipoprotein B-100 increases the atherogenicity of low-density lipoproteins (LDL). However, little is known about the potential oxidative damage to apolipoprotein E (apoE), an exchangeable antiatherogenic apolipoprotein. ApoE plays an integral role in lipoprotein metabolism by regulating the plasma cholesterol and triglyceride levels. Hepatic uptake of lipoproteins is facilitated by apoE's ability to bind with cell surface heparan sulfate proteoglycans and to lipoprotein receptors via basic residues in its 22 kDa N-terminal domain (NT). We investigated the effect of acrolein, an aldehydic product of endogenous lipid peroxidation and a tobacco smoke component, on the conformation and function of recombinant human apoE3-NT. Acrolein caused oxidative modification of apoE3-NT as detected by Western blot with acrolein-lysine-specific antibodies, and tertiary conformational alterations. Acrolein modification impairs the ability of apoE3-NT to interact with heparin and the LDL receptor. Furthermore, acrolein-modified apoE3-NT displayed a 5-fold decrease in its ability to interact with lipid surfaces. Our data indicate that acrolein disrupts the functional integrity of apoE3, which likely interferes with its role in regulating plasma cholesterol homeostasis. These observations have implications regarding the role of apoE in the pathogenesis of smoking- and oxidative stress-mediated cardiovascular and cerebrovascular diseases.
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Registered trials
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