ArticlePhysiological genomics2007
Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumber Holothuria glaberrima.
Article in Physiological genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 63 citations in OpenAlex.
- Sea CucumberResearch square · 2026Article
- Article
- Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium.bioRxiv : the preprint server for biology · 2024Article
- EchinoDB: an update to the web-based application for genomic and transcriptomic data on echinoderms.BMC genomic data · 2022Article
- Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea CucumberMarine drugs · 2022Article
- A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians.Biology · 2022Review
- Characterization of Two Novel EF-Hand Proteins Identifies a Clade of Putative CaJournal of bioinformatics and systems biology : Open access · 2022Article
- Distinct microbiota assembly mechanisms revealed in different reconstruction stages during gut regeneration in the sea cucumber Apostichopus japonicus.MicrobiologyOpen · 2021Article
- Article
- Review
- Review
- A roadmap for intestinal regeneration.The International journal of developmental biology · 2021Review
- Draft Genome of the Sea CucumberFrontiers in Marine Science · 2021Article
- Regeneration in Echinoderms: Molecular Advancements.Frontiers in cell and developmental biology · 2021Review
- Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes.Frontiers in cell and developmental biology · 2020Review
- Retinoic Acid Signaling Is Associated with Cell Proliferation, Muscle Cell Dedifferentiation, and Overall Rudiment Size during Intestinal Regeneration in the Sea Cucumber,Biomolecules · 2019Article
- The mesentery as the epicenter for intestinal regeneration.Seminars in cell & developmental biology · 2019Review
- Article
- Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers.Frontiers in physiology · 2018Article
- Melanotransferrin: New Homolog Genes and Their Differential Expression during Intestinal Regeneration in the Sea Cucumber Holothuria glaberrima.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Repair and regeneration are key processes for tissue maintenance, and their disruption may lead to disease states. Little is known about the molecular mechanisms that underline the repair and regeneration of the digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model to dissect and characterize the molecular events during intestinal regeneration. To study the gene expression profile, cDNA libraries were constructed from normal, 3-day, and 7-day regenerating intestines of H. glaberrima. Clones were randomly sequenced and queried against the nonredundant protein database at the National Center for Biotechnology Information. RT-PCR analyses were made of several genes to determine their expression profile during intestinal regeneration. A total of 5,173 sequences from three cDNA libraries were obtained. About 46.2, 35.6, and 26.2% of the sequences for the normal, 3-days, and 7-days cDNA libraries, respectively, shared significant similarity with known sequences in the protein database of GenBank but only present 10% of similarity among them. Analysis of the libraries in terms of functional processes, protein domains, and most common sequences suggests that a differential expression profile is taking place during the regeneration process. Further examination of the expressed sequence tag dataset revealed that 12 putative genes are differentially expressed at significant level (R > 6). Experimental validation by RT-PCR analysis reveals that at least three genes (unknown C-4677-1, melanotransferrin, and centaurin) present a differential expression during regeneration. These findings strongly suggest that the gene expression profile varies among regeneration stages and provide evidence for the existence of differential gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.