ArticleProceedings of the National Academy of Sciences of the United States of America2007
Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 71 citations in OpenAlex.
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- Non-Tuberculous Mycobacteria: Molecular and Physiological Bases of Virulence and Adaptation to Ecological Niches.Microorganisms · 2020Review
- Global Assessment of Mycobacterium avium subsp.mSystems · 2019Article
- Characterization of membrane vesicles released by Mycobacterium avium in response to environment mimicking the macrophage phagosome.Future microbiology · 2019Article
- Divalent Metal Cations Potentiate the Predatory Capacity of Amoeba for Cryptococcus neoformans.Applied and environmental microbiology · 2018Article
- Discovery and Characterization ofFrontiers in microbiology · 2018Article
- MAP1203 PromotesFrontiers in cellular and infection microbiology · 2018Article
- Dual Role of Mechanisms Involved in Resistance to Predation by Protozoa and Virulence to Humans.Frontiers in microbiology · 2018Review
- The Voltage-Dependent Anion Channels (VDAC) of Mycobacterium avium phagosome are associated with bacterial survival and lipid export in macrophages.Scientific reports · 2017Article
- Mycobacterium llatzerense, a waterborne Mycobacterium, that resists phagocytosis by Acanthamoeba castellanii.Scientific reports · 2017Article
- Article
- Identification of Mycobacterium avium subsp. hominissuis secreted proteins using an in vitro system mimicking the phagosomal environment.BMC microbiology · 2016Article
- Identification of Prophages within theAdvances in microbiology · 2016Article
Corrections and comments
- Erratum issuedCirillo, Stuart [corrected to Cirillo, Suat L G]; Cirillo, Jeffrey [corrected to Cirillo, Jeffrey D]
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to infect macrophages is a common characteristic shared among many mycobacterial species. Mycobacterium avium, Mycobacterium tuberculosis, and Mycobacterium kansasii enter macrophages, using the complement receptors CR1, CR3, CR4, and the mannose receptor. To identify M. avium genes and host cell pathways involved in the bacterial uptake by macrophages, we screened a M. avium transposon mutant library for the inability to enter macrophages. Uptake-impaired clones were selected. Sequence of six M. avium clones identified one gene involved in glycopeptidolipid biosynthesis, one gene encoding the conserved membrane protein homologue to the M. avium subsp. paratuberculosis MAP2446c gene and four others belonging to the same region of the chromosome. Analysis of the chromosome region revealed a pathogenicity island inserted between two tRNA sequences with 58% of G+C content versus 69% in the M. avium genome. The region is unique for M. avium and is not present in M. tuberculosis or M. paratuberculosis. Although the mutants did not differ from the WT bacterium regarding the binding to macrophage cell membrane, analysis of macrophage proteins after 1 h infection revealed a deficiency in the mutant to phosphorylate certain proteins on uptake. To understand M. avium interaction with two evolutionarily distinct hosts, the mutants were evaluated for Acanthamoeba castellanii invasion. The defect in the ability of the mutants to invade both cells was highly similar, suggesting that M. avium might have evolved mechanisms that are used to enter amoebas and human macrophages.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.