ArticleIn vitro cellular & developmental biology. Animal
Establishment and characterization of a primary canine duodenal epithelial cell culture.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Characterization of the inflammatory response of a canine intestinal epithelial cell line challenged with lipopolysaccharides and/or butyrate.Journal of animal science · 2026Article
- Ki-67/CD3 ratio in the diagnosis of chronic inflammatory enteropathy in dogs.Journal of veterinary internal medicine · 2020Article
- Methodology for the establishment of primary porcine vocal fold epithelial cell cultures.The Laryngoscope · 2019Article
- Establishment of immortalized mouse intestinal epithelial cells line and study of effects of Arg-Arg on inflammatory response.In vitro cellular & developmental biology. Animal · 2017Article
- Primary cultures of mouse small intestinal epithelial cells using the dissociating enzyme type I collagenase and hyaluronidase.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line.PloS one · 2014Article
- Establishment of an in vitro culture system for intestinal epithelial cells from Pheretima aspergillum (E. Perrier).In vitro cellular & developmental biology. Animal · 2014Article
- Generation of functional insulin-producing cells in the gut by Foxo1 ablation.Nature genetics · 2012Article
- Article
- Characterization of newly established bovine intestinal epithelial cell line.Histochemistry and cell biology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many mechanisms involved in the pathogenesis of chronic enteropathies or host-pathogen interactions in canine intestine have not been elucidated so far. Next to the clinical and in vivo research tools, an in vitro model of canine intestinal cell culture would be very helpful for studies at the cellular level. Therefore, the purpose of this study was to establish and characterize a primary canine duodenal epithelial cell culture. Neonatal duodenum was disrupted with trypsin-ethylenediaminetetraacetic acid (EDTA) and the mucosa scraped off and digested with collagenase and dispase. After centrifugation on a 2% sorbitol gradient, the cells were incubated at 37 degrees C in OptiMEM supplemented with Primocin, epidermal growth factor, insulin, hydrocortisone, and 10% fetal calf serum (FCS). After 24 h, the FCS concentration was reduced to 2.5%, and the temperature decreased to 33 degrees C. With this method, the cultures were growing to confluent monolayers within 5-6 d and remained viable for an average of 2 wk. Their epithelial nature was confirmed by electron microscopy and immunofluorescence staining using antibodies directed against specific cytokeratins, desmosomes, and tight junctions. The intestinal cells proliferated, as evidenced by immunolabeling with a Ki-67 antibody, and cryptal cell subpopulations could be identified. Furthermore, alkaline phosphatase and sucrase activity were detected.
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