ArticleThe Journal of cell biology2007
Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed.
- Anaphase onset requires CKS-1-mediated destruction of securin in meiosis I and cyclin B1 in meiosis II.The Journal of cell biology · 2026Article
- Anaphase B: Long-standing models meet new concepts.Seminars in cell & developmental biology · 2021Review
- Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.eLife · 2021Article
- Ase1 domains dynamically slow anaphase spindle elongation and recruit Bim1 to the midzone.Molecular biology of the cell · 2020Article
- Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.PLoS genetics · 2020Article
- Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics.The Journal of cell biology · 2018Article
- Theoretical model of mitotic spindle microtubule growth for FRAP curve interpretation.BMC systems biology · 2017Article
- Anaphase B.Biology · 2016Review
- Understanding force-generating microtubule systems through in vitro reconstitution.Cell adhesion & migration · 2016Review
- Mechanisms of Mitotic Spindle Assembly.Annual review of biochemistry · 2016Review
- Mechanism for Anaphase B: Evaluation of "Slide-and-Cluster" versus "Slide-and-Flux-or-Elongate" Models.Biophysical journal · 2015Article
- The microtubule cross-linker Feo controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos.Molecular biology of the cell · 2015Article
- KIF4A and PP2A-B56 form a spatially restricted feedback loop opposing Aurora B at the anaphase central spindle.The Journal of cell biology · 2014Article
- Modelling chromosome dynamics in mitosis: a historical perspective on models of metaphase and anaphase in eukaryotic cells.Interface focus · 2014Review
- Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation.The Journal of cell biology · 2013Article
- A single Drosophila embryo extract for the study of mitosis ex vivo.Nature protocols · 2013Article
- Simplified equation to extract diffusion coefficients from confocal FRAP data.Traffic (Copenhagen, Denmark) · 2012Article
- The emergence of sarcomeric, graded-polarity and spindle-like patterns in bundles of short cytoskeletal polymers and two opposite molecular motors.Journal of physics. Condensed matter : an Institute of Physics journal · 2011Article
- KIF4 regulates midzone length during cytokinesis.Current biology : CB · 2011Article
- Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles.Cell division · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Anaphase B in Drosophila embryos is initiated by the inhibition of microtubule (MT) depolymerization at spindle poles, which allows outwardly sliding interpolar (ip) MTs to drive pole-pole separation. Using fluorescence recovery after photobleaching, we observed that MTs throughout the preanaphase B spindle are very dynamic and display complete recovery of fluorescence, but during anaphase B, MTs proximal to the poles stabilize and therefore display lower recovery than those elsewhere. Fluorescence microscopy of the MT tip tracker EB1 revealed that growing MT plus ends localize throughout the preanaphase B spindle but concentrate in the overlap region of interpolar MTs (ipMTs) at anaphase B onset. None of these changes occurred in the presence of nondegradable cyclin B. Modeling suggests that they depend on the establishment of a spatial gradient of MT plus-end catastrophe frequencies, decreasing toward the equator. The resulting redistribution of ipMT plus ends to the overlap zone, together with the suppression of minus-end depolymerization at the poles, could constitute a mechanical switch that initiates spindle elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.