ArticleNucleic acids research2007
Different structural states in oligonucleosomes are required for early versus late steps of base excision repair.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- Base excision repair in chromatin: A tug-of-war for DNA damage.DNA repair · 2025Review
- Article
- The chromatin remodeler ERCC6 and the histone chaperone NAP1 are involved in apurinic/apyrimidinic endonuclease-mediated DNA repair.The Plant cell · 2024Article
- Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Obstacles and opportunities for base excision repair in chromatin.DNA repair · 2022Review
- Chaperoning histones at the DNA repair dance.DNA repair · 2021Review
- Resolving DNA Damage: Epigenetic Regulation of DNA Repair.Molecules (Basel, Switzerland) · 2020Review
- Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress.PLoS pathogens · 2020Article
- Targeting DNA repair in cancer: current state and novel approaches.Cellular and molecular life sciences : CMLS · 2020Review
- Acetylation of the histone H3 tail domain regulates base excision repair on higher-order chromatin structures.Scientific reports · 2019Article
- Initiating base excision repair in chromatin.DNA repair · 2018Review
- Article
- Unencumbered Pol β lyase activity in nucleosome core particles.Nucleic acids research · 2017Article
- Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes.DNA repair · 2017Article
- The Human Ligase IIIα-XRCC1 Protein Complex Performs DNA Nick Repair after Transient Unwrapping of Nucleosomal DNA.The Journal of biological chemistry · 2017Article
- A basic domain in the histone H2B N-terminal tail is important for nucleosome assembly by FACT.Nucleic acids research · 2016Article
- Facilitation of base excision repair by chromatin remodeling.DNA repair · 2015Review
- Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair.DNA repair · 2015Review
- Base excision repair: a critical player in many games.DNA repair · 2014Review
- Chromatin remodelling complex RSC promotes base excision repair in chromatin of Saccharomyces cerevisiae.DNA repair · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Chromatin in eukaryotic cells is folded into higher order structures of folded nucleosome filaments, and DNA damage occurs at all levels of this structural hierarchy. However, little is known about the impact of higher order folding on DNA repair enzymes. We examined the catalytic activities of purified human base excision repair (BER) enzymes on uracil-containing oligonucleosome arrays, which are folded primarily into 30 nm structures when incubated in repair reaction buffers. The catalytic activities of uracil DNA glycosylase (UDG) and apyrimidinic/apurinic endonuclease (APE) digest G:U mismatches to completion in the folded oligonucleosomes without requiring significant disruption. In contrast, DNA polymerase beta (Pol beta) synthesis is inhibited in a major fraction ( approximately 80%) of the oligonucleosome array, suggesting that single strand nicks in linker DNA are far more accessible to Pol beta in highly folded oligonucleosomes. Importantly, this barrier in folded oligonucleosomes is removed by purified chromatin remodeling complexes. Both ISW1 and ISW2 from yeast significantly enhance Pol beta accessibility to the refractory nicked sites in oligonucleosomes. These results indicate that the initial steps of BER (UDG and APE) act efficiently on highly folded oligonucleosome arrays, and chromatin remodeling may be required for the latter steps of BER in intact chromatin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.