ArticleNucleic acids research2007
A novel endonuclease activity associated with the Arabidopsis ortholog of the 30-kDa subunit of cleavage and polyadenylation specificity factor.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 77 citations in OpenAlex.
- The non-tandem CCCH zinc finger protein AtC3H26 modulates salt stress response and phosphate starvation response through its ribonuclease activity in Arabidopsis.BMC plant biology · 2025Article
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- Functional Analysis of CPSF30 inInsects · 2024Article
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- Genome-Wide Identification and Evolutionary Analysis ofPlants (Basel, Switzerland) · 2023Article
- Natural variation in the plant polyadenylation complex.Frontiers in plant science · 2023Article
- Article
- Genome-Wide Investigation of N6-Methyladenosine Regulatory Genes and Their Roles in Tea (Frontiers in plant science · 2021Article
- Characterization of UVA-Induced Alterations to Transfer RNA Sequences.Biomolecules · 2020Article
- Novel Aspects of Nitrate Regulation inFrontiers in plant science · 2020Review
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- N6-methyladenosine regulatory machinery in plants: composition, function and evolution.Plant biotechnology journal · 2019Review
- Molecular Regulation of Nitrate Responses in Plants.International journal of molecular sciences · 2018Review
- Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in cucumber (Cucumis sativus).Genes & genomics · 2018Article
- The YTH Domain Protein ECT2 Is an mThe Plant cell · 2018Article
- Article
- Article
- Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF.The Journal of biological chemistry · 2016Article
- Molecular characterization of HbCZF1, a Hevea brasiliensis CCCH-type zinc finger protein that regulates hmg1.Plant cell reports · 2015Article
- CPSF30 at the Interface of Alternative Polyadenylation and Cellular Signaling in Plants.Biomolecules · 2015Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polyadenylation of messenger RNAs is mediated by a multi-subunit complex that is conserved in eukaryotes. Among the most interesting of these proteins is the 30-kDa-subunit of the Cleavage and Polyadenylation Specificity Factor, or CPSF30. In this study, the Arabidopsis CPSF30 ortholog, AtCPSF30, is characterized. This protein possesses an unexpected endonucleolytic activity that is apparent as an ability to nick and degrade linear as well as circular single-stranded RNA. Endonucleolytic action by AtCPSF30 leaves RNA 3' ends with hydroxyl groups, as they can be labeled by RNA ligase with [32P]-cytidine-3',5'-bisphosphate. Mutations in the first of the three CCCH zinc finger motifs of the protein abolish RNA binding by AtCPSF30 but have no discernible effects on nuclease activity. In contrast, mutations in the third zinc finger motif eliminate the nuclease activity of the protein, and have a modest effect on RNA binding. The N-terminal domain of another Arabidopsis polyadenylation factor subunit, AtFip1(V), dramatically inhibits the nuclease activity of AtCPSF30 but has a slight negative effect on the RNA-binding activity of the protein. These results indicate that AtCPSF30 is a probable processing endonuclease, and that its action is coordinated through its interaction with Fip1.
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