ArticleAmerican journal of respiratory cell and molecular biology2007
Development of lentiviral vectors with regulated respiratory epithelial expression in vivo.
Article in American journal of respiratory cell and molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Gene Therapeutics for Surfactant Dysfunction Disorders: Targeting the Alveolar Type 2 Epithelial Cell.Human gene therapy · 2022Review
- Targeted Type 2 Alveolar Cell Depletion. A Dynamic Functional Model for Lung Injury Repair.American journal of respiratory cell and molecular biology · 2016Article
- ERK5 negatively regulates tobacco smoke-induced pulmonary epithelial-mesenchymal transition.Oncotarget · 2015Article
- Lentivirus vector-mediated Rho guanine nucleotide dissociation inhibitor 2 induces beta-2 adrenergic receptor desensitization in β2AR desensitization mice model.Journal of thoracic disease · 2014Article
- Regulation of lung injury and fibrosis by p53-mediated changes in urokinase and plasminogen activator inhibitor-1.The American journal of pathology · 2013Article
- Article
- The a"MAZE"ing world of lung-specific transgenic mice.American journal of respiratory cell and molecular biology · 2012Review
- Genetic modification of airway progenitors after lentiviral gene delivery to the amniotic fluid of murine fetuses.American journal of respiratory cell and molecular biology · 2011Article
- Clinical review: gene-based therapies for ALI/ARDS: where are we now?Critical care (London, England) · 2011Review
- Jaagsiekte sheep retrovirus biology and oncogenesis.Viruses · 2010Article
- Lentiviral vectors and cystic fibrosis gene therapy.Viruses · 2010Article
- Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages.The Journal of clinical investigation · 2010Article
- Lentiviral transduction of the murine lung provides efficient pseudotype and developmental stage-dependent cell-specific transgene expression.Gene therapy · 2008Article
- Specific in vivo expression in type II pneumocytes of the Jaagsiekte sheep retrovirus long terminal repeat in transgenic mice.Virology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Development of gene transfer vectors with regulated, lung-specific expression will be a useful tool for studying lung biology and developing gene therapies. In this study we constructed a series of lentiviral vectors with regulatory elements predicted to produce lung-specific transgene expression: the surfactant protein C promoter (SPC) for alveolar epithelial type II cell (AECII) expression, the Clara cell 10-kD protein (CC10) for Clara cell expression in the airway, and the Jaagskiete sheep retrovirus (JSRV) promoter for expression in both cell types. Transgene expression from the SPC and CC10 vectors was restricted to AECII and Clara cell lines, respectively, while expression from the JSRV vector was observed in multiple respiratory and nonrespiratory cell types. After intratracheal delivery of lentivector supernatant to mice, transgene expression was observed in AECII from the SPC lentivector, and in Clara cells from the CC10-promoted lentivector. Transgene expression was not detected in nonrespiratory tissues after intravenous delivery of CC10 and SPC lentiviral vectors to murine recipients. In summary, incorporation of genomic regulatory elements from the SPC and CC10 genes resulted in respiratory specific transgene expression in vitro and in vivo. These vectors will provide a useful tool for the study of lung biology and the development of gene therapies for lung disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.