ArticleApplied and environmental microbiology2007
Transposon insertion reveals pRM, a plasmid of Rickettsia monacensis.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
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- Examination of Rickettsial Host Range for Shuttle Vectors Based onApplied and environmental microbiology · 2022Article
- Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle.Nature reviews. Microbiology · 2021Review
- Isolation and characterization of a Rickettsia from the ovary of a Western black-legged tick, Ixodes pacificus.Ticks and tick-borne diseases · 2019Article
- The Wolbachia mobilome in Culex pipiens includes a putative plasmid.Nature communications · 2019Article
- GFPuv-Expressing Recombinant Rickettsia typhi: a Useful Tool for the Study of Pathogenesis and CD8Infection and immunity · 2017Article
- Fluorescent Protein Expressing Rickettsia buchneri and Rickettsia peacockii for Tracking Symbiont-Tick Cell Interactions.Veterinary sciences · 2016Article
- The Wolbachia WO bacteriophage proteome in the Aedes albopictus C/wStr1 cell line: evidence for lytic activity?In vitro cellular & developmental biology. Animal · 2016Article
- Electrotransformation and Clonal Isolation of Rickettsia Species.Current protocols in microbiology · 2015Article
- A Rickettsia genome overrun by mobile genetic elements provides insight into the acquisition of genes characteristic of an obligate intracellular lifestyle.Journal of bacteriology · 2012Article
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- Advances in genetic manipulation of obligate intracellular bacterial pathogens.Frontiers in microbiology · 2011Article
- Rickettsia felis infection in a common household insect pest, Liposcelis bostrychophila (Psocoptera: Liposcelidae).Applied and environmental microbiology · 2010Article
- Wide dispersal and possible multiple origins of low-copy-number plasmids in rickettsia species associated with blood-feeding arthropods.Applied and environmental microbiology · 2010Article
- Rickettsial ompB promoter regulated expression of GFPuv in transformed Rickettsia montanensis.PloS one · 2010Article
- Conjugation genes are common throughout the genus Rickettsia and are transmitted horizontally.Proceedings. Biological sciences · 2009Article
- Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reduction.BMC genomics · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Until the recent discovery of pRF in Rickettsia felis, the obligate intracellular bacteria of the genus Rickettsia (Rickettsiales: Rickettsiaceae) were thought not to possess plasmids. We describe pRM, a plasmid from Rickettsia monacensis, which was detected by pulsed-field gel electrophoresis and Southern blot analyses of DNA from two independent R. monacensis populations transformed by transposon-mediated insertion of coupled green fluorescent protein and chloramphenicol acetyltransferase marker genes into pRM. Two-dimensional electrophoresis showed that pRM was present in rickettsial cells as circular and linear isomers. The 23,486-nucleotide (31.8% G/C) pRM plasmid was cloned from the transformant populations by chloramphenicol marker rescue of restriction enzyme-digested transformant DNA fragments and PCR using primers derived from sequences of overlapping restriction fragments. The plasmid was sequenced. Based on BLAST searches of the GenBank database, pRM contained 23 predicted genes or pseudogenes and was remarkably similar to the larger pRF plasmid. Two of the 23 genes were unique to pRM and pRF among sequenced rickettsial genomes, and 4 of the genes shared by pRM and pRF were otherwise found only on chromosomes of R. felis or the ancestral group rickettsiae R. bellii and R. canadensis. We obtained pulsed-field gel electrophoresis and Southern blot evidence for a plasmid in R. amblyommii isolate WB-8-2 that contained genes conserved between pRM and pRF. The pRM plasmid may provide a basis for the development of a rickettsial transformation vector.
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