Evidence map›Paper›PMID 17574992›Full record

ArticleApplied and environmental microbiology2007

Essential role of cytochrome bd-related oxidase in cyanide resistance of Pseudomonas pseudoalcaligenes CECT5344.

Alberto Quesada, M Isabel Guijo, Faustino Merchán, Blas Blázquez, M Isabel Igeño, Rafael Blasco

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.3field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Characterization of a Periplasmic D-Malate:CytochromeInternational journal of molecular sciences · 2025
    Article
  3. Genomic Insights into Cyanide Biodegradation in theInternational journal of molecular sciences · 2024
    Article
  4. Article
  5. The cryoEM structure of cytochromeFrontiers in chemistry · 2022
    Article
  6. Article
  7. Mechanistic and structural diversity between cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  8. Article
  9. Article
  10. Article
  11. Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading BacteriumInternational journal of molecular sciences · 2019
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. The cytochrome bd respiratory oxygen reductases.Biochimica et biophysica acta · 2011
    Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Alberto QuesadaDepartamento de Bioquímica, Biología Molecular y Genética, Facultad de Veterinaria, Avda. de la Universidad s/n, 10071-Cáceres, España. aquesada@unex.es
M Isabel Guijo
Faustino Merchán
Blas Blázquez
M Isabel Igeño
Rafael Blasco
Universidad de Extremadura · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pseudomonas pseudoalcaligenes CECT5344 grows in minimal medium containing cyanide as the sole nitrogen source. Under these conditions, an O2-dependent respiration highly resistant to cyanide was detected in cell extracts. The structural genes for the cyanide-resistant terminal oxidase, cioA and cioB, are clustered and encode the integral membrane proteins that correspond to subunits I and II of classical cytochrome bd, although the presence of heme d in the membrane could not be detected by difference spectra. The cio operon from P. pseudoalcaligenes presents a singular organization, starting upstream of cioAB by the coding sequence of a putative ferredoxin-dependent sulfite or nitrite reductase and spanning downstream two additional open reading frames that encode uncharacterized gene products. PCR amplifications of RNA (reverse transcription-PCR) indicated the cyanide-dependent up-regulation and cotranscription along the operon. The targeted disruption of cioA eliminates both the expression of the cyanide-stimulated respiratory activity and the growth with cyanide as the nitrogen source, which suggests a critical role of this cytochrome bd-related oxidase in the metabolism of cyanide by P. pseudoalcaligenes CECT5344.

Indexed as

Drug Resistance, BacterialBacterial ProteinsChromosome MappingCloning, MolecularCyanidesGene Expression Regulation, BacterialGene OrderModels, GeneticMolecular Sequence DataMutagenesisOperonOxidoreductasesPseudomonas pseudoalcaligenesReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNABacterial ProteinsCyanidesOxidoreductases

Identifiers

PMID17574992
PMCPMC1950984
OpenAlexW2130689433

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.