ArticleThe Journal of cell biology1991
The actin filament severing protein actophorin promotes the formation of rigid bundles of actin filaments crosslinked with alpha-actinin.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 89 citations in OpenAlex.
- Correlation between chemical denaturation and the unfolding energetics of Acanthamoeba actophorin.Biophysical journal · 2023Article
- Nonlinear Microscale Mechanics of Actin Networks Governed by Coupling of Filament Crosslinking and Stabilization.Polymers · 2022Article
- Structure and activity of a thermally stable mutant of Acanthamoeba actophorin.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Unravelling the Biology of EhActo as the First Cofilin FromFrontiers in cell and developmental biology · 2022Article
- Filament-guided filament assembly provides structural memory of filament alignment during cytokinesis.Developmental cell · 2021Article
- Fascin-induced actin protrusions are suppressed by dendritic networks in giant unilamellar vesicles.Molecular biology of the cell · 2021Article
- Article
- In vitro efficacies of clinically available drugs against growth and viability of an Acanthamoeba castellanii keratitis isolate belonging to the T4 genotype.Antimicrobial agents and chemotherapy · 2013Article
- α-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly.Molecular biology of the cell · 2012Article
- Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring.The Journal of cell biology · 2011Article
- Cofilin cooperates with fascin to disassemble filopodial actin filaments.Journal of cell science · 2011Article
- Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover.Biophysical journal · 2010Article
- The regulatory action of alpha-actinin on actin filaments is enhanced by cofilin.European biophysics journal : EBJ · 2010Article
- Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.The open neuroscience journal · 2009Article
- Alpha-actinin is required for tightly regulated remodeling of the actin cortical network during cytokinesis.Developmental cell · 2007Article
- UNC-87, a calponin-related protein in C. elegans, antagonizes ADF/cofilin-mediated actin filament dynamics.Journal of cell science · 2007Article
- Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba.The Korean journal of parasitology · 2006Article
- Regulation of the pollen-specific actin-depolymerizing factor LlADF1.The Plant cell · 2002Article
- Actin-binding proteins in the Arabidopsis genome database: properties of functionally distinct plant actin-depolymerizing factors/cofilins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Review
- VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations.Cell motility and the cytoskeleton · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The actin filament severing protein, Acanthamoeba actophorin, decreases the viscosity of actin filaments, but increases the stiffness and viscosity of mixtures of actin filaments and the crosslinking protein alpha-actinin. The explanation of this paradox is that in the presence of both the severing protein and crosslinker the actin filaments aggregate into an interlocking meshwork of bundles large enough to be visualized by light microscopy. The size of these bundles depends on the size of the containing vessel. The actin filaments in these bundles are tightly packed in some areas while in others they are more disperse. The bundles form a continuous reticulum that fills the container, since the filaments from a particular bundle may interdigitate with filaments from other bundles at points where they intersect. The same phenomena are seen when rabbit muscle aldolase rather than alpha-actinin is used as the crosslinker. We propose that actophorin promotes bundling by shortening the actin filaments enough to allow them to rotate into positions favorable for lateral interactions with each other via alpha-actinin. The network of bundles is more rigid and less thixotropic than the corresponding network of single actin filaments linked by alpha-actinin. One explanation may be that alpha-actinin (or aldolase) normally in rapid equilibria with actin filaments may become trapped between the filaments increasing the effective concentration of the crosslinker.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.