ArticleMolecular microbiology2007
An atypical receiver domain controls the dynamic polar localization of the Myxococcus xanthus social motility protein FrzS.
Article in Molecular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
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- Combinatorial control of type IVa pili formation by the four polarized regulators MglA, SgmX, FrzS, and SopA.Journal of bacteriology · 2024Article
- FrzS acts as a polar beacon to recruit SgmX, a central activator of type IV pili during Myxococcus xanthus motility.The EMBO journal · 2023Article
- To ∼P or Not to ∼P? Non-canonical activation by two-component response regulators.Molecular microbiology · 2017Review
- Evolution and Design Governing Signal Precision and Amplification in a Bacterial Chemosensory Pathway.PLoS genetics · 2015Article
- The aspartate-less receiver (ALR) domains: distribution, structure and function.PLoS pathogens · 2015Article
- Article
- VirS, an OmpR/PhoB subfamily response regulator, is required for activation of vapA gene expression in Rhodococcus equi.BMC microbiology · 2014Article
- Response regulator heterodimer formation controls a key stage in Streptomyces development.PLoS genetics · 2014Article
- Response to metronidazole and oxidative stress is mediated through homeostatic regulator HsrA (HP1043) in Helicobacter pylori.Journal of bacteriology · 2014Article
- Atypical response regulator ChxR from Chlamydia trachomatis is structurally poised for DNA binding.PloS one · 2014Article
- Mutations to essential orphan response regulator HP1043 of Helicobacter pylori result in growth-stage regulatory defects.Infection and immunity · 2013Article
- Structure of the pilus assembly protein TadZ from Eubacterium rectale: implications for polar localization.Molecular microbiology · 2012Article
- The atypical response regulator protein ChxR has structural characteristics and dimer interface interactions that are unique within the OmpR/PhoB subfamily.The Journal of biological chemistry · 2011Article
- The atypical OmpR/PhoB response regulator ChxR from Chlamydia trachomatis forms homodimers in vivo and binds a direct repeat of nucleotide sequences.Journal of bacteriology · 2011Article
- FrzS regulates social motility in Myxococcus xanthus by controlling exopolysaccharide production.PloS one · 2011Article
- Regulation of response regulator autophosphorylation through interdomain contacts.The Journal of biological chemistry · 2010Article
- Role of acetyl-phosphate in activation of the Rrp2-RpoN-RpoS pathway in Borrelia burgdorferi.PLoS pathogens · 2010Article
- Gliding motility revisited: how do the myxobacteria move without flagella?Microbiology and molecular biology reviews : MMBR · 2010Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The Myxococcus xanthus FrzS protein transits from pole-to-pole within the cell, accumulating at the pole that defines the direction of movement in social (S) motility. Here we show using atomic-resolution crystallography and NMR that the FrzS receiver domain (RD) displays the conserved switch Tyr102 in an unusual conformation, lacks the conserved Asp phosphorylation site, and fails to bind Mg(2+) or the phosphoryl analogue, Mg(2+) x BeF(3). Mutation of Asp55, closest to the canonical site of RD phosphorylation, showed no motility phenotype in vivo, demonstrating that phosphorylation at this site is not necessary for domain function. In contrast, the Tyr102Ala and His92Phe substitutions on the canonical output face of the FrzS RD abolished S-motility in vivo. Single-cell fluorescence microscopy measurements revealed a striking mislocalization of these mutant FrzS proteins to the trailing cell pole in vivo. The crystal structures of the mutants suggested that the observed conformation of Tyr102 in the wild-type FrzS RD is not sufficient for function. These results support the model that FrzS contains a novel 'pseudo-receiver domain' whose function requires recognition of the RD output face but not Asp phosphorylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.