ArticleJournal of bacteriology2007
Conjugal transfer of chromosomal DNA contributes to genetic variation in the oral pathogen Porphyromonas gingivalis.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
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- Article
- Phylogenetic diversity inJournal of oral microbiology · 2020Article
- Immunological Pathways Triggered byMediators of inflammation · 2019Review
- Gene expression of Porphyromonas gingivalis ATCC 33277 when growing in an in vitro multispecies biofilm.PloS one · 2019Article
- Community Development betweenmBio · 2018Article
- Genomic repeats, misassembly and reannotation: a case study with long-read resequencing of Porphyromonas gingivalis reference strains.BMC genomics · 2018Article
- Genetic exchange and reassignment inJournal of oral microbiology · 2018Review
- Insights into Dynamic Polymicrobial Synergy Revealed by Time-Coursed RNA-Seq.Frontiers in microbiology · 2017Article
- Genes Contributing toFrontiers in cellular and infection microbiology · 2017Article
- Investigation of potential targets of Porphyromonas CRISPRs among the genomes of Porphyromonas species.PloS one · 2017Article
- Porphyromonas gingivalis initiates a mesenchymal-like transition through ZEB1 in gingival epithelial cells.Cellular microbiology · 2016Article
- Draft Genome Sequence of Low-Passage Clinical Isolate Porphyromonas gingivalis MP4-504.Genome announcements · 2016Article
- Functional Advantages of Porphyromonas gingivalis Vesicles.PloS one · 2015Article
- Expression of Porphyromonas gingivalis small RNA in response to hemin availability identified using microarray and RNA-seq analysis.FEMS microbiology letters · 2014Article
- The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome.Frontiers in cellular and infection microbiology · 2014Review
- Genetic exchange of fimbrial alleles exemplifies the adaptive virulence strategy of Porphyromonas gingivalis.PloS one · 2014Article
- Design of a multiple drug delivery system directed at periodontitis.Biomaterials · 2013Article
- Genetic diversity in the oral pathogen Porphyromonas gingivalis: molecular mechanisms and biological consequences.Future microbiology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Porphyromonas gingivalis is a major oral pathogen that contributes to the development of periodontal disease. There is a significant degree of genetic variation among strains of P. gingivalis, and the population structure has been predicted to be panmictic, indicating that horizontal DNA transfer and recombination between strains are likely. The molecular events underlying this genetic exchange are not understood, although a putative type IV secretion system is present in the genome sequence of strain W83, implying that DNA conjugation may be responsible for genetic transfer in these bacteria. In this study, we provide in vitro evidence for the horizontal transfer of DNA using plasmid- and chromosome-based assays. In the plasmid assays, Bacteroides-derived shuttle vectors were tested for transfer from P. gingivalis strains into Escherichia coli. Of the eight strains tested, five were able to transfer DNA into E. coli by a mechanism most consistent with conjugation. Additionally, strains W83 and 33277 tested positive for the transfer of chromosomally integrated antibiotic resistance markers. Ten chimeras resulting from the chromosomal transfer assay were further analyzed by Southern hybridization and were shown to have exchanged DNA fragments of between 1.1 and 5.6 kb, but the overall strain identity remained intact. Chimeras showed phenotypic changes in the ability to accrete into biofilms, implying that DNA transfer events are sufficient to generate measurable changes in complex behaviors. This ability to transfer chromosomal DNA between strains may be an adaptation mechanism in the complex environment of the host oral cavity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.