ArticleBiophysical journal2007
Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime imaging.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Lipidomic profiling reveals age-dependent changes in plasma membrane lipids that affect neural stem cell aging.Science advances · 2026Article
- Enhanced Chemical Sensing with Multiorder Coherent Raman Scattering Spectroscopic Dephasing.Analytical chemistry · 2022Article
- Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.Membranes · 2021Article
- Influence of Membrane Phase on the Optical Properties of DPH.Molecules (Basel, Switzerland) · 2020Article
- Principles and Applications of Biological Membrane Organization.Annual review of biophysics · 2020Review
- Photophysics and Cell Uptake of Self-Assembled Ru(II)Polypyridyl Vesicles.Frontiers in chemistry · 2020Article
- Fluorescence lifetime imaging of membrane lipid order with a ratiometric fluorescent probe.Biophysical journal · 2015Article
- A surface-bound molecule that undergoes optically biased Brownian rotation.Nature nanotechnology · 2014Article
- A transparent organic transistor structure for bidirectional stimulation and recording of primary neurons.Nature materials · 2013Article
- Dynamics and size of cross-linking-induced lipid nanodomains in model membranes.Biophysical journal · 2012Article
- Development of fluorophore dynamics imaging as a probe for lipid domains in model vesicles and cell membranes.European biophysics journal : EBJ · 2011Article
- Fluorescence lifetime measurements and biological imaging.Chemical reviews · 2010Review
- Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2010Article
- Watching conformational- and photo-dynamics of single fluorescent proteins in solution.Nature chemistry · 2010Article
- Combining fluorescence lifetime and polarization microscopy to discriminate phase separated domains in giant unilamellar vesicles.Biophysical journal · 2008Article
- Detection of lipid domains in model and cell membranes by fluorescence lifetime imaging microscopy of fluorescent lipid analogues.The Journal of biological chemistry · 2008Article
- Amino(oligo)thiophene-based environmentally sensitive biomembrane chromophores.The Journal of organic chemistry · 2008Article
Corrections and comments
- Erratum issuedVallée, Renaud A L [added]
Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new membrane probe, based on the perylene imide chromophore, with excellent photophysical properties (high absorption coefficient, quantum yield (QY) approximately 1, high photostability) and excited in the visible domain is proposed for the study of membrane rafts. Visualization of separation between the liquid-ordered (Lo) and the liquid-disordered (Ld) phases can be achieved in artificial membranes by fluorescence lifetime imaging due to the different decay times of the membrane probe in the two phases. Rafts on micrometer-scale in cell membranes due to cellular activation can also be observed by this method. The decay time of the dye in the Lo phase is higher than in organic solvents where its QY is 1. This allows proposing a (possible general) mechanism for the decay time increase in the Lo phase, based on the local field effects of the surrounding molecules. For other fluorophores with QY<1, the suggested mechanism could also contribute, in addition to effects reducing the nonradiative decay pathways, to an increase of the fluorescence decay time in the Lo phase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.