ArticleNature chemical biology2007
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator.
Article in Nature chemical biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 191 citations in OpenAlex.
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- PAD4 and Its Inhibitors in Cancer Progression and Prognosis.Pharmaceutics · 2022Review
- Bioorthogonal, Fluorogenic Targeting of Voltage-Sensitive Fluorophores for Visualizing Membrane Potential Dynamics in Cellular Organelles.Journal of the American Chemical Society · 2022Article
- The virtues and vices of protein citrullination.Royal Society open science · 2022Review
- Fluorescent and Bioluminescent Calcium Indicators with Tuneable Colors and Affinities.Journal of the American Chemical Society · 2022Article
- Dual-locked spectroscopic probes for sensing and therapy.Nature reviews. Chemistry · 2021Review
- Optical tools for understanding the complexity of β-cell signalling and insulin release.Nature reviews. Endocrinology · 2018Review
- Gap Junctions in the Nervous System: Probing Functional Connections Using New Imaging Approaches.Frontiers in cellular neuroscience · 2018Review
- Rotamer-Restricted Fluorogenicity of the Bis-Arsenical ReAsH.Journal of the American Chemical Society · 2016Article
- Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors.ACS nano · 2016Article
- Fast kinetics of calcium signaling and sensor design.Current opinion in chemical biology · 2015Review
- Chemical biology of protein arginine modifications in epigenetic regulation.Chemical reviews · 2015Review
- Protein arginine deiminase 2 binds calcium in an ordered fashion: implications for inhibitor design.ACS chemical biology · 2015Article
- Ion channel engineering: perspectives and strategies.Journal of molecular biology · 2015Review
- Accounting for randomness in measurement and sampling in studying cancer cell population dynamics.IET systems biology · 2014Article
- Separating the isomers--efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B.Bioorganic & medicinal chemistry letters · 2014Article
- Fluorescent sensors for measuring metal ions in living systems.Chemical reviews · 2014Review
- Regulation of hemichannels and gap junction channels by cytokines in antigen-presenting cells.Mediators of inflammation · 2014Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Intracellular Ca(2+) regulates numerous proteins and cellular functions and can vary substantially over submicron and submillisecond scales, so precisely localized fast detection is desirable. We have created a approximately 1-kDa biarsenical Ca(2+) indicator, called Calcium Green FlAsH (CaGF, 1), to probe [Ca(2+)] surrounding genetically targeted proteins. CaGF attached to a tetracysteine motif becomes ten-fold more fluorescent upon binding Ca(2+), with a K(d) of approximately 100 microM, <1-ms kinetics and good Mg(2+) rejection. In HeLa cells expressing tetracysteine-tagged connexin 43, CaGF labels gap junctions and reports Ca(2+) waves after injury. Total internal reflection microscopy of tetracysteine-tagged, CaGF-labeled alpha(1C) L-type calcium channels shows fast-rising depolarization-evoked Ca(2+) transients, whose lateral nonuniformity suggests that the probability of channel opening varies greatly over micron dimensions. With moderate Ca(2+) buffering, these transients decay surprisingly slowly, probably because most of the CaGF signal comes from closed channels feeling Ca(2+) from a tiny minority of clustered open channels. With high Ca(2+) buffering, CaGF signals decay as rapidly as the calcium currents, as expected for submicron Ca(2+) domains immediately surrounding active channels. Thus CaGF can report highly localized, rapid [Ca(2+)] dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.