ArticleFolia microbiologica2007
Single multiplex polymerase chain reaction for environmental surveillance of toxigenic-pathogenic O1 and non-O1 Vibrio cholerae.
Article in Folia microbiologica, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 48 citations in OpenAlex.
- A Label-Free Colorimetric AuNP-Aptasensor for the Rapid Detection ofCellular and molecular bioengineering · 2024Article
- Advances in phage display based nano immunosensors for cholera toxin.Frontiers in immunology · 2023Review
- Epidemiologic potentials and correlational analysis of Vibrio species and virulence toxins from water sources in greater Bushenyi districts, Uganda.Scientific reports · 2021Article
- Molecular characterization ofIranian journal of microbiology · 2021Article
- Simultaneous Quantification ofPathogens (Basel, Switzerland) · 2020Article
- Characterisation ofInternational journal of microbiology · 2020Article
- Vibrio chemaguriensis sp. nov., from Sundarbans, Bay of Bengal.Current microbiology · 2019Article
- Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa.International journal of environmental research and public health · 2017Article
- High genetic diversity of Vibrio cholerae in the European lake Neusiedler See is associated with intensive recombination in the reed habitat and the long-distance transfer of strains.Environmental microbiology · 2017Article
- A novel triplex quantitative PCR strategy for quantification of toxigenic and nontoxigenic Vibrio cholerae in aquatic environments.Applied and environmental microbiology · 2015Article
- Isolation, characterization, and antibiotic resistance of Vibrio spp. in sea turtles from Northwestern Mexico.Frontiers in microbiology · 2015Article
- Faecal pollution loads in the wastewater effluents and receiving water bodies: a potential threat to the health of Sedibeng and Soshanguve communities, South Africa.Environmental science and pollution research international · 2014Article
- Detection of ctx gene positive non-O1/non-O139 V. cholerae in shrimp aquaculture environments.Journal of food science and technology · 2013Article
- Molecular characterization of Vibrio cholerae outbreak strains with altered El Tor biotype from southern India.World journal of microbiology & biotechnology · 2010Article
- Characterization of Vibrio cholerae from deep ground water in a cholera endemic area in Central India.Indian journal of microbiology · 2009Article
- RNA colony blot hybridization method for enumeration of culturable Vibrio cholerae and Vibrio mimicus bacteria.Applied and environmental microbiology · 2009Article
- Detection of virulence genes in environmental strains of Vibrio cholerae from estuaries in northeastern Brazil.Revista do Instituto de Medicina Tropical de Sao PauloArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex PCR assay was developed for the detection of toxigenic and pathogenic V. cholerae from direct water sources using specific primers targeting diverse genes, viz. outer membrane protein (ompW), cholera toxin (ctxB), ORF specific for O1 (rfbG), zonula occludens (zot) and toxin co-regulated pilus (tcpB); among these genes, ompW acts as internal control for V. cholerae, the ctx gene as a marker for toxigenicity and tcp for pathogenicity. The sensitivity of multiplex PCR was 5 x 10(4) V. cholerae cells per reaction. The procedure was simplified as direct bacterial cells were used as template and there was no need for DNA extraction. The assay was specific as no amplification occurred with the other bacteria used. Toxigenic V. cholerae were artificially spiked in different water samples, filtered through a 0.45 microm membrane, and the filters containing bacteria were enriched in APW for 6 h. PCR following filtration and enrichment could detect as little as 8 V. cholerae cells per mL in different spiked water samples. Various environmental potable water samples were screened for the presence of V. cholerae using this assay procedure. The proposed method is rapid, sensitive and specific for environmental surveillance for the presence of toxigenic-pathogenic and nonpathogenic V. cholerae.
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