ArticleBMC microbiology2007
Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
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- The PrfA regulon ofMicrobiology (Reading, England) · 2024Article
- Transcriptomic Analysis ofFrontiers in microbiology · 2021Article
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- Virulence assessment of Listeria monocytogenes grown in different foods using a Galleria mellonella model.PloS one · 2020Article
- Oxygen deprivation influences the survival ofTranslational animal science · 2019Article
- TheFrontiers in microbiology · 2018Article
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- Food-Associated Stress Primes Foodborne Pathogens for the Gastrointestinal Phase of Infection.Frontiers in microbiology · 2018Review
- Metabolic determinants in Listeria monocytogenes anaerobic listeriolysin O production.Archives of microbiology · 2017Article
- Tim-3 inhibits macrophage control of Listeria monocytogenes by inhibiting Nrf2.Scientific reports · 2017Article
- Influence of pH on bile sensitivity amongst various strains of Listeria monocytogenes under aerobic and anaerobic conditions.Journal of medical microbiology · 2015Article
- Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Growth on Vacuum-Packed Cold Smoked Salmon.Applied and environmental microbiology · 2015Article
- Oxygen limitation induces acid tolerance and impacts simulated gastro-intestinal transit in Listeria monocytogenes J0161.Gut pathogens · 2015Article
- Listeria monocytogenes: survival and adaptation in the gastrointestinal tract.Frontiers in cellular and infection microbiology · 2014Review
- Acid shock of Listeria monocytogenes at low environmental temperatures induces prfA, epithelial cell invasion, and lethality towards Caenorhabditis elegans.BMC genomics · 2013Article
- A comparative analysis of the intestinal metagenomes present in guinea pigs (Cavia porcellus) and humans (Homo sapiens).BMC genomics · 2012Article
- Implications of the inability of Listeria monocytogenes EGD-e to grow anaerobically due to a deletion in the class III NrdD ribonucleotide reductase for its use as a model laboratory strain.Journal of bacteriology · 2011Article
- Growth temperature-dependent contributions of response regulators, σB, PrfA, and motility factors to Listeria monocytogenes invasion of Caco-2 cells.Foodborne pathogens and disease · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundListeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxygen restriction, was compared in vitro and in vivo. Fluorescent protein labels were applied to allow certain identification of Listeria cells from untagged bacteria in in vivo samples, and to distinguish between cells grown under different conditions in mixed infection experiments.
resultsInfection of Caco-2 cells revealed that Listeria cultivated under oxygen-restricted conditions were approximately 100 fold more invasive than similar cultures grown without oxygen restriction. This was observed for exponentially growing bacteria, as well as for stationary-phase cultures. Oral dosage of guinea pigs with Listeria resulted in a significantly higher prevalence (p < 0.05) of these bacteria in jejunum, liver and spleen four and seven days after challenge, when the bacterial cultures had been grown under oxygen-restricted conditions prior to dosage. Additionally, a 10-100 fold higher concentration of Listeria in fecal samples was observed after dosage with oxygen-restricted bacteria. These differences were seen after challenge with single Listeria cultures, as well as with a mixture of two cultures grown with and without oxygen restriction.
conclusionOur results show for the first time that the environmental conditions to which L. monocytogenes is exposed prior to ingestion are decisive for its in vivo infective potential in the gastrointestinal tract after passage of the gastric barrier. This is highly relevant for safety assessment of this organism in food.
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