Evidence map›Paper›PMID 17570012›Full record

ArticleCurrent microbiology2007

Influence of lipopolysaccharide on the surface proton-binding behavior of Shewanella spp.

Vernon R Phoenix, Anton A Korenevsky, F G Ferris, Y A Gorby, T J Beveridge

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 14 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Vernon R PhoenixDepartment of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph, N1G 2W1, Ontario, Canada. Vernon.Phoenix@ges.gla.ac.uk
Anton A Korenevsky
F G Ferris
Y A Gorby
T J Beveridge
University of Guelph · CAUniversity of Toronto · CAJ. Craig Venter Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigates the potentiometric properties of several strains of Shewanella spp. and determines whether these properties can be correlated with lipopolysaccharide (LPS) type. The LPS of eight Shewanella strains was characterized using silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and their potentiometric properties determined using high-resolution acid-base titrations. Titrations showed that total ligand concentrations (L(T)) ranged from 0.903 +/- 0.007 micromol/mg (S. baltica 63) to 1.387 +/- 0.007 micromol/mg (S. amazonensis SB2B). Smooth strains (possessing O-side chains) exhibited higher mean L(T) values than rough strains (no O-side chain). A Tukey's honestly significantly different (HSD) test revealed, smooth strains exhibited significantly higher L(T) values than rough strains in 69% of comparisons. Comparison of individual pK(a) concentrations revealed that smooth LPS strains of Shewanella were relatively enriched in reactive groups at pK(a) 5, suggesting their LPS O-side chains contained detectable carboxyl groups. Combined pKa spectra from all eight Shewanella strains produced a common trend indicating that the way in which the surface proton-buffering capacity changes with pH is similar for the species studied here.

Indexed as

Cell MembraneHydrogen-Ion ConcentrationLigandsLipopolysaccharidesPotentiometryProtonsShewanellaLigandsLipopolysaccharidesProtons

Identifiers

PMID17570012
OpenAlexW2080951539

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.